纳米硒对甜菜叶片转录组的影响

doi:10.7668/hbnxb.20194488

摘要/Abstract

摘要：

为揭示喷施外源硒对甜菜响应的分子机制,采用糖甜菜品系HD802种子作为供试材料进行水培试验,纳米硒浓度分别设置为0(喷施清水,对照),20,50,80,100,150,200 mg/L,待真叶长至8片叶时均匀地喷施纳米硒溶液,24 h后取样进行超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量测定。经数据分析,选取50,150 mg/L的纳米硒处理叶片送检,进行转录组测定,分析差异表达基因和显著富集通路。结果表明,纳米硒50 mg/L处理对甜菜叶片生长具有促进作用,而150 mg/L处理对甜菜叶片具有破坏效果。鉴定出9 161个DEGs,其中,上调表达3 717个、下调表达5 444个;GO功能富集主要富集在信号转导、细胞通讯、膜的组成部分、膜的固有成分、初级代谢过程、有机物代谢过程和生物过程等;KEGG代谢途径主要包括植物病原互作、植物激素信号转导等途径;转录因子分析共涉及AP2、zf-Dof、HLH、WRKY、HSF_DNA-bind、NAM、zf-BED、Homeobox等11个家族。研究找到了硒处理对甜菜幼苗生长发育有促进作用的适宜浓度,初步筛选出甜菜外源硒响应的相关基因。

关键词: 甜菜, 纳米硒, RNA-Seq, 差异基因, 代谢通路

Abstract:

In order to reveal the molecular mechanism of the response to exogenous selenium of sugar beet,seeds of the sugar beet line HD802 were used as test materials for hydroponic experiments.The concentrations of nano-selenium were set to 0(water spray,control),20,50,80,100,150,200 mg/L,respectively.When the plants grew to 8 true leaves stage,nano-selenium solution was evenly sprayed.After 24 h,the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and the content of malondialdehyde(MDA)were measured.After data analysis,50,150 mg/L nano-selenium treated leaves were selected for examination.RNA-Seq was performed to analyze the differentially expressed genes and significant enrichment pathways.The results showed that 50 mg/L nano-selenium treatment had promoting effects on the growth of sugar beet leaves,while 150 mg/L treatment had destructive effects on sugar beet leaves.A total of 9 161 DEGs were identified,of which 3 717 were up-regulated and 5 444 were down-regulated.GO functional enrichment was mainly enriched in processes such as signal transduction,cell communication, integral component of membrane, intrinsic component of membrane, primary metabolic process, organic substance metabolic process, and biological process. The metabolic pathways of KEGG mainly included plant pathogen interaction,plant hormone signal transduction,and other pathways.Transcription factor analysis involved 11 families,including AP2,zf-Dof,HLH,WRKY,HSF_DNA-bind,NAM,zf-BED and Homeobox.It found the optimal concentration for selenium treatment to promote the growth and development of sugar beet seedlings,and preliminarily screened the relevant genes responsive to exogenous selenium in sugar beet.

Key words: Sugar beet, Nano-selenium, RNA-Seq, Differential gene, Metabolic pathway

郑文哲, 王莹莹, 张辉, 张必周, 孙梦媛, 王良, 张惠忠, 李晓东, 付增娟, 赵尚敏, 鄂圆圆, 张自强. 纳米硒对甜菜叶片转录组的影响[J]. 华北农学报, 2024, 39(3): 51-59. doi: 10.7668/hbnxb.20194488.

ZHENG Wenzhe, WANG Yingying, ZHANG Hui, ZHANG Bizhou, SUN Mengyuan, WANG Liang, ZHANG Huizhong, LI Xiaodong, FU Zengjuan, ZHAO Shangmin, E Yuanyuan, ZHANG Ziqiang. Effects of Nano-selenium on the Transcriptome of Sugar Beet Leaves[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 51-59. doi: 10.7668/hbnxb.20194488.

http://www.hbnxb.net/CN/Y2024/V39/I3/51

图/表 6

参考文献 38

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样品 Sample	原始读段/条 Raw reads	有效读段/条 Clean reads	过滤后碱基/Gb Clean bases	错误率/% Error rate	Q30/%
CK-1	51 915 302	51 909 586	7.73	0.04	92.05
CK-2	52 796 436	52 790 504	7.87	0.04	92.83
CK-3	57 862 260	57 856 624	8.64	0.04	92.02
150 mg/L-1	36 209 686	36 206 182	5.39	0.04	91.12
150 mg/L-2	41 834 274	41 830 462	6.23	0.04	92.07
150 mg/L-3	40 405 984	40 402 760	6.02	0.04	89.72
50 mg/L-1	42 093 642	42 088 736	6.27	0.04	93.39
50 mg/L-2	41 264 466	41 260 298	6.14	0.04	92.84
50 mg/L-3	37 532 788	37 528 802	5.59	0.04	91.94

样品 Sample	原始读段/条 Raw reads	有效读段/条 Clean reads	过滤后碱基/Gb Clean bases	错误率/% Error rate	Q30/%
CK-1	51 915 302	51 909 586	7.73	0.04	92.05
CK-2	52 796 436	52 790 504	7.87	0.04	92.83
CK-3	57 862 260	57 856 624	8.64	0.04	92.02
150 mg/L-1	36 209 686	36 206 182	5.39	0.04	91.12
150 mg/L-2	41 834 274	41 830 462	6.23	0.04	92.07
150 mg/L-3	40 405 984	40 402 760	6.02	0.04	89.72
50 mg/L-1	42 093 642	42 088 736	6.27	0.04	93.39
50 mg/L-2	41 264 466	41 260 298	6.14	0.04	92.84
50 mg/L-3	37 532 788	37 528 802	5.59	0.04	91.94

样品 Sample	有效读段/条 Clean reads	所有能比对上的读段/条 (比例/%) Total mapped	唯一比对读段/条 (比例/%) Unique mapped	多比对读段/条(比例/%) Multiple mapped
CK-1	51 909 586	45 368 129(87.40)	40 348 502(77.73)	5 019 627(9.67)
CK-2	52 790 504	46 227 390(87.57)	39 536 828(74.89)	6 690 562(12.67)
CK-3	57 856 624	50 414 842(87.14)	43 496 329(75.18)	6 918 513(11.96)
150 mg/L-1	36 206 182	31 235 013(86.27)	23 271 117(64.27)	7 963 896(22.00)
150 mg/L-2	41 830 462	36 189 429(86.51)	26 865 816(64.23)	9 323 613(22.29)
150 mg/L-3	40 402 760	34 627 031(85.70)	26 259 083(64.99)	8 367 948(20.71)
50mg/L-1	42 088 736	35 996 234(85.52)	25 276 476(60.06)	10 719 758(25.47)
50 mg/L-2	41 260 298	35 137 623(85.16)	27 329 554(66.24)	7 808 069(18.92)
50 mg/L-3	37 528 802	32 247 102(85.93)	25 521 134(68.00)	6 725 968(17.92)

样品 Sample	有效读段/条 Clean reads	所有能比对上的读段/条 (比例/%) Total mapped	唯一比对读段/条 (比例/%) Unique mapped	多比对读段/条(比例/%) Multiple mapped
CK-1	51 909 586	45 368 129(87.40)	40 348 502(77.73)	5 019 627(9.67)
CK-2	52 790 504	46 227 390(87.57)	39 536 828(74.89)	6 690 562(12.67)
CK-3	57 856 624	50 414 842(87.14)	43 496 329(75.18)	6 918 513(11.96)
150 mg/L-1	36 206 182	31 235 013(86.27)	23 271 117(64.27)	7 963 896(22.00)
150 mg/L-2	41 830 462	36 189 429(86.51)	26 865 816(64.23)	9 323 613(22.29)
150 mg/L-3	40 402 760	34 627 031(85.70)	26 259 083(64.99)	8 367 948(20.71)
50mg/L-1	42 088 736	35 996 234(85.52)	25 276 476(60.06)	10 719 758(25.47)
50 mg/L-2	41 260 298	35 137 623(85.16)	27 329 554(66.24)	7 808 069(18.92)
50 mg/L-3	37 528 802	32 247 102(85.93)	25 521 134(68.00)	6 725 968(17.92)

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