干旱锻炼对小麦幼苗期抗氧化特性的影响

doi:10.7668/hbnxb.20192270

华北农学报 ›› 2021, Vol. 36 ›› Issue (S1): 125-129. doi: 10.7668/hbnxb.20192270

所属专题： 小麦；抗旱节水；

• 耕作栽培·生理生化 • 上一篇下一篇

干旱锻炼对小麦幼苗期抗氧化特性的影响

万雪洁¹, 张艳², 师长海¹, 周宣材¹, 孙新灵¹, 李松坚³, 张守福⁴, 刘义国¹

1. 青岛农业大学, 山东省旱作农业技术重点实验室旱作节水创新团队, 山东青岛 266109;
2. 济宁市兖州区农业农村局, 山东兖州 272100;
3. 青岛市农业技术推广中心, 山东青岛 266071;
4. 青岛海力源生物科技有限公司, 山东青岛 266000

收稿日期:2021-04-21 出版日期:2021-12-28
通讯作者: 刘义国(1979-),男,山东临朐人,副教授,博士,主要从事小麦栽培理论与技术研究。
作者简介:万雪洁(1989-),女,山东烟台人,讲师,博士,主要从事小麦栽培生理研究。
基金资助:
山东省农业重大应用技术创新项目（SD2019ZZ003）；山东省重点研发计划（2019GNC106012）；山东省高等学校青创科技支持计划（2019KJF013）；青岛农业大学高层次人才科研基金（6631114308）；青岛市科技惠民引导专项（21-1-4-ny-31-nsh）

Priming of Drought on the Antioxidant Characteristics of Wheat Seedlings

WAN Xuejie¹, ZHANG Yan², SHI Changhai¹, ZHOU Xuancai¹, SUN Xinling¹, LI Songjian³, ZHANG Shoufu⁴, LIU Yiguo¹

1. Qingdao Agricultural University, Shandong Province Key Laboratory of Dry Farming Technology, Dry Land Crop and Water Innovation Team, Qingdao 266109, China;
2. Agriculture and Rural Bureau of Yanzhou District, Yanzhou 272100, China;
3. Qingdao Agricultural Technology Extension Center, Qingdao 266071, China;
4. Qingdao Hailiyuan Biotechnology Limited Company, Qingdao 266000, China

Received:2021-04-21 Published:2021-12-28

摘要/Abstract

摘要： 为探究干旱锻炼对小麦幼苗期抗氧化特性的影响，设置对照处理和干旱锻炼处理，在一心一叶时用PEG6000营养液进行干旱锻炼，之后恢复霍格兰氏全营养液，在三叶一心时分别进行干旱胁迫处理，观测幼叶中与抗氧化特性相关物质的动态变化。研究发现，随干旱程度及胁迫时间的增加，叶片SOD、POD活性、可溶性蛋白不同程度的减小，MDA、可溶性糖含量显著升高；与对照相比，干旱锻炼后叶片SOD、POD活性、可溶性蛋白及降低程度较小，可溶性糖含量升高程度较大，MDA升高程度较小；不同干旱程度下，25% PEG6000胁迫下干旱锻炼小麦的抗氧化能力较35% PEG6000胁迫下较高。由此说明，小麦经过一定浓度的干旱锻炼能够明显增强其对干旱环境的适应能力，有利于幼苗期的生长，也为小麦的抗逆性研究提供理论支持。

关键词: 小麦, 幼苗期, 干旱锻炼, 抗氧化特性

Abstract: In order to explore the effects of drought priming on antioxidant properties of wheat seedlings, control treatment and drought training treatment were set up in this experiment. PEG6000 nutrient solution was used for drought training when one heart and one leaf, and then Hoagland's total nutrient solution was restored. When three leaves are one heart, the drought stress is treated separately, and the dynamic changes of the substances related to the antioxidant properties in the young leaves were observed. The results showed that with the increase of drought degree and stress time, SOD, POD activity and soluble protein decreased, but MDA and soluble sugar content increased significantly. Compared with the control, the leaf SOD, POD activity and soluble protein decreased less after drought exercise, the soluble sugar content increased more, and the MDA increased less. Under the different drought stress, the antioxidative capacity of drought-stressed wheat under 25% PEG6000 stress was higher than that of 35% PEG6000. This showed that a certain concentration of drought training in wheat can significantly enhance its ability to adapt to drought conditions, which was beneficial to the growth of the seedling stage and provides theoretical support for the research on wheat resistance.

Key words: Wheat, Seedling stage, Drought priming, Antioxidant properties

中图分类号:

S512.01

万雪洁, 张艳, 师长海, 周宣材, 孙新灵, 李松坚, 张守福, 刘义国. 干旱锻炼对小麦幼苗期抗氧化特性的影响[J]. 华北农学报, 2021, 36(S1): 125-129. doi: 10.7668/hbnxb.20192270.

WAN Xuejie, ZHANG Yan, SHI Changhai, ZHOU Xuancai, SUN Xinling, LI Songjian, ZHANG Shoufu, LIU Yiguo. Priming of Drought on the Antioxidant Characteristics of Wheat Seedlings[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 125-129. doi: 10.7668/hbnxb.20192270.

http://www.hbnxb.net/CN/Y2021/V36/IS1/125

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