象草叶片和茎秆木质素合成相关基因的表达模式分析

doi:10.7668/hbnxb.20190867

摘要/Abstract

摘要： 为了系统地研究象草茎叶中木质素合成相关基因的表达模式，以N51象草为试验材料，测定了象草茎秆倒数第一、第三、第五节间以及倒数第一、第三、第五、第七和第九叶片的木质素含量及相关合成基因的表达量。结果表明，在成熟象草中，木质素含量在茎和叶垂直的空间结构中由上到下逐级递增。实时荧光定量PCR表达分析显示，不同木质素合成基因的表达表现出了组织特异性，叶组织的COMT、C3H和HCT4基因表达量显著低于茎组织，如COMT在倒五节间的表达量最高，约为叶片中表达量的6倍，而4CL和F5H基因在叶组织中却显著高于茎组织，如4CL在倒九叶的表达量最高，约为倒一节间表达量的14倍。关联分析结果表明，茎秆中的木质素含量与F5H、HCT4以及CCoAOMT基因的表达呈正相关，与C3H基因的表达呈负相关，而叶片中的木质素含量与4CL基因的表达呈正相关，C4H、COMT、HCT4以及CCR基因的表达呈负相关。该结果可以说明不同的木质素合成酶在不同的组织中呈现表达差异，并且在不同组织中，合成的主要木质素也不同。

关键词: 象草, 木质素合成, 木质素单体, 实时荧光定量PCR, 基因表达

Abstract: In order to systematically study the expression changes of lignin biosynthesis genes in the stems and leaves of elephant grass,the lignin content and lignin biosynthesis gene expressions were determined in the first,third and fifth stem internodes,as well as the top first,third,fifth,seventh and ninth leaf of N51 elephant grass. The contents of lignin in stem internodes and leaves increased from the head to the base of the mature elephant grass. Real-time quantitative PCR analysis showed that the expression of different lignin biosynthesis genes was tissue-specific. The expression of COMT, C3H and HCT4 genes in leaf tissue was significantly lower than that in stem tissues. For example, COMT had the highest expression level in the fifth internode,which was about 6 times that in leaves. The 4CL and F5H gene expressions were significantly higher than those in the stem tissues. For example, 4CL had the highest expression level in the ninth leaf,which was about 14 times the expression level of the first internode. Correlation analysis showed that the lignin content in stems was positively correlated with the expression of F5H, HCT4 and CCoAOMT genes,and negatively correlated with the expression of C3H gene,while the lignin content in leaves was positively correlated with the expression of 4CL gene,and negatively correlated with the expression of C4H, COMT, HCT4 and CCR genes. This result indicated that different lignin synthetases showed obvious expression differences in different tissues,and the main lignin synthesized was also different in different tissues.

Key words: Elephant grass, Lignin biosynthesis, Lignol, Real-time fluorescence quantitative PCR, Gene expression

中图分类号:

付程程, 黄淮安, 周梦蝶, 钟天秀, 张向前, 陈曙, 解新明. 象草叶片和茎秆木质素合成相关基因的表达模式分析[J]. 华北农学报, 2020, 35(4): 87-95. doi: 10.7668/hbnxb.20190867.

FU Chengcheng, HUANG Huaian, ZHOU Mengdie, ZHONG Tianxiu, ZHANG Xiangqian, CHEN Shu, XIE Xinming. Expression Analysis of Lignin Biosynthesis Genes of Pennisetum purpureum Leaf and Stem[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 87-95. doi: 10.7668/hbnxb.20190867.

http://www.hbnxb.net/CN/Y2020/V35/I4/87

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