外源GA<sub>3</sub>和PBZ对桃枝条生长及其GA相关基因表达的影响

doi:10.7668/hbnxb.201751523

摘要/Abstract

摘要： 为探究外源GA₃和PBZ处理对黄水蜜桃新梢生长及赤霉素合成代谢和信号转导途径相关基因表达的影响。对1年生黄水蜜桃枝条进行外源GA₃和PBZ喷施处理，测定新梢生长量，并利用qRT-PCR对GA合成代谢和信号传导途径中8个相关基因的表达量进行测定。结果表明：外源GA₃处理后新梢净生长量在处理8 d时显著高于对照，GA合成代谢相关基因KO和GA3-ox的表达量呈现出处理前期先升高后降低，处理后期逐渐升高的趋势，而GA信号转导相关基因DELLA、GID1c、SLY1的表达量呈现出在处理前期受抑制，后期迅速升高的趋势，且在处理后14 d时表达量达到峰值；外源PBZ处理后新梢净生长量在多个时期显著低于对照，并在处理后17 d时达极显著水平，GA合成代谢相关基因KO、GA20-ox、GA2-ox的表达量在整个处理过程中呈现出先逐渐升高随后降低的趋势，而GA3-ox基因在处理后整个时期近乎不表达，GA信号转导相关基因DELLA、GID1c、SLY1、ERF11的表达量在整个处理过程中均略低于对照，推测PBZ处理在转录水平上通过抑制活性GA的合成进而影响GA信号转导途径相关基因的表达来调节植物生长；外源GA₃逆转处理能显著解除PBZ对植物生长的抑制作用，且信号转导相关基因GID1c的表达量呈现出先升高后降低的趋势，DELLA基因的表达呈现出大多时期被抑制的情况。

关键词: 桃, 赤霉素, 多效唑, 新梢长度, 逆转处理, 基因表达

Abstract: To investigate the effects of exogenous GA₃ and PBZ treatments on the growth of shoots and the expression of genes related to gibberellin biosynthesis and gibberellin signal transduction pathway in Huangshuimi, the annual shoots of Huangshuimi were treated with exogenous GA₃ and PBZ. The growth of shoots was measured and the expression levels of eight GA-related genes were determined by qRT-PCR. The results showed that the net length of shoots at 8 d was significantly higher in GA₃ treatment than in CK. The expressions of KO and GA3-ox showed a trend of firstly increasing and then decreasing in the early stage after treatment, while gradually rising in the later stages. The expression of DELLA, GID1c and SLY1 genes related to GA signal transduction was inhibited at the early stage and then rapidly increased in the late growth period after treatment. And the expression of the three genes was up to maximum in 14 d after treatment. For the PBZ treatment, the net length of shoots was lower than the CK at many stages, and reached to a significant level at 17 d after treatment. The expression of genes related to gibberellin biosynthesis KO, GA20-ox and GA2-ox showed the trend of increasing gradually and then decreased. While GA3-ox showed almost no expression in whole treatment period. The expression of DELLA, GID1c, SLY1 and ERF11 related to gibberellin signal transduction pathway were slightly lower than the CK during the whole process. It was speculated that the exogenous PBZ might regulate the plant growth at the transcriptional level by inhibiting the synthesis of active gibberellin and further affecting the expression of genes involved in the gibberellin signal transduction pathway. Meanwhile, the reverse treatment of GA₃ could significantly abolish the inhibitory effect of PBZ on plant growth, and the expression of GID1c gene involved in gibberellin signal transduction pathway increased firstly and then decreased under GA₃ treatment, while the expression of DELLA gene appeared to be inhibited in most of the time.

Key words: Peach, Gibberellin, Paclobutrazol, The length of the shoot, Reverse treatment, Gene expression

中图分类号:

S662.03
Q78

谭彬, 王婷, 郝鹏博, 郑先波, 程钧, 王伟, 冯建灿. 外源GA₃和PBZ对桃枝条生长及其GA相关基因表达的影响[J]. 华北农学报, 2019, 34(2): 25-34. doi: 10.7668/hbnxb.201751523.

TAN Bin, WANG Ting, HAO Pengbo, ZHENG Xianbo, CHENG Jun, WANG Wei, FENG Jiancan. Effects of GA₃ and PBZ on Shoot Growth and Expression of GA-related Genes in Peach[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 25-34. doi: 10.7668/hbnxb.201751523.

http://www.hbnxb.net/CN/Y2019/V34/I2/25

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外源GA₃和PBZ对桃枝条生长及其GA相关基因表达的影响

Effects of GA₃ and PBZ on Shoot Growth and Expression of GA-related Genes in Peach

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外源GA3和PBZ对桃枝条生长及其GA相关基因表达的影响

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外源GA₃和PBZ对桃枝条生长及其GA相关基因表达的影响

Effects of GA₃ and PBZ on Shoot Growth and Expression of GA-related Genes in Peach