华北农学报 ›› 2013, Vol. 28 ›› Issue (5): 101-105. doi: 10.7668/hbnxb.2013.05.018

所属专题: 玉米

• 论文 • 上一篇    下一篇

玉米自交系08-641不同选择方向回交改良后代的遗传变异

李芦江1, 熊尧宇1,2, 陈文生3, 兰海1, 林海建1, 杨克诚1, 高世斌1, 潘光堂1   

  1. 1 四川农业大学玉米研究所 农业部西南玉米生物学与遗传育种重点实验室 四川 雅安 625014;
    2 四川省成都市新津县农村发展局 四川 成都 611430;
    3 四川农业大学农学院 四川 成都 611130
  • 收稿日期:2013-07-19 出版日期:2013-10-28
  • 通讯作者: 杨克诚(1940-),男,四川郫县人,教授,主要从事玉米遗传育种研究。
  • 作者简介:李芦江(1982-),男,四川芦山人,助理研究员,博士,主要从事玉米遗传育种研究。
  • 基金资助:
    国家玉米产业技术体系项目(CARS-02-07);“十二五”农村领域国家科技计划课题项目(2011AA10A103-2);四川省教育厅资助科研项目(13ZA0250)

The Genetic Variation of the Backcross Modified Lines Developed from the Maize Line 08-641 Selected by Different Directions

LI Lu-jiang1, XIONG Yao-yu1,2, CHEN Wen-sheng3, LAN Hai1, LIN Hai-jian1, YANG Ke-cheng1, GAO Shi-bin1, PAN Guang-tang1   

  1. 1 Maize Research Institute, Sichuan Agricultural University, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Ya'an 625014, China;
    2 Xinjin Rural Development Burean, Chengdu 611430, China;
    3 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2013-07-19 Published:2013-10-28

摘要: 利用SSR分子标记对玉米自交系08-641(R08)不同选择方向回交改良得到的79个BC2F4后代的遗传多样性进行分析。结果表明,利用44对具有清晰多态性的引物共检测到272个多态性等位基因位点,其中有123个位点在回交后代株系中被检测到而在轮回亲本中缺失;以种子形状和颜色不像R08为标准选择得到的回交后代株系,基因型与R08的相似程度总体低于以种子形状和颜色类似R08为标准选择得到的回交后代株,且其变异范围更广。可见不同选择方向所得回交后代选系的遗传变异存在一定的差异。因此,在回交过程中采用多向选择的策略,可能有利于提高回交改良的育种效率。

关键词: 玉米, 自交系, 回交, 选择方向, 08-641, 遗传变异, SSR

Abstract: In this study,SSR molecular markers were used to analysis the genetic variation of 79 backcross modi-fied lines of the maize inbred line 08-641(R08).The result showed that a total of 272 alleles were detected in the im-proved lines and R08,and 123 of them were detected in the modified lines but discarded in R 08.The modified line se-lected based on simialry in the shape and corlor of seeds to R 08 have lower genetic similary between R 08 than that between the lines selected based on dissimialry in the shape and corlor of seeds and R 08,and the genetic varioation of theses lines were wider. It conduvted that when the backcross breeding were used to improve the maize inbred lines, multidirectional selection based on phenotypic value were contribute to create and keep genetic variation.

Key words: Maize, Inbred lines, Backcross breeding, Select direction, 08-641, Genetic variation, SSR

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引用本文

李芦江, 熊尧宇, 陈文生, 兰海, 林海建, 杨克诚, 高世斌, 潘光堂. 玉米自交系08-641不同选择方向回交改良后代的遗传变异[J]. 华北农学报, 2013, 28(5): 101-105. doi: 10.7668/hbnxb.2013.05.018.

LI Lu-jiang, XIONG Yao-yu, CHEN Wen-sheng, LAN Hai, LIN Hai-jian, YANG Ke-cheng, GAO Shi-bin, PAN Guang-tang. The Genetic Variation of the Backcross Modified Lines Developed from the Maize Line 08-641 Selected by Different Directions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 101-105. doi: 10.7668/hbnxb.2013.05.018.

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