摘要： 水稻稻瘟病是最具毁灭性的病害之一,严重地影响水稻的高产和稳产。在病原菌侵染水稻时,附着胞的形成对稻瘟病菌的致病性起着关键作用。研究证实一种P型ATP酶(P-ATPase)参与了附着胞的形成。在病原菌与寄主的互作过程中,寄主的一些小分子物质可以进入病原菌中,达到抗病原菌侵染的目的。以稻瘟菌致病关键的P-ATPase基因MgAPT2第一外显子上特异性好的232 bp的区域作为干扰片段,正反向插入干扰载体中,通过农杆菌介导,转化到感稻瘟病水稻品种日本晴中,通过苗期稻瘟病接种鉴定和MgAPT2基因的表达检测,结果表明:转基因植株稻瘟病抗性得到增强且稻瘟病菌MgAPT2基因的表达量下降,为水稻抗稻瘟病种质资源的创新提供了新思路。

关键词: 水稻, 抗稻瘟病, 基因沉默

Abstract: Rice blast disease is the severest disease in rice production. The formation of appressorium plays a key role on disease extending when pathogenic fungi intrudes into rice. The formation of appressorium needs a P-type ATPase. Small molecules from the host are able to enter into the pathogen during the interactions between pathogen and host,to reach the purpose of anti-pathogen infection. In this study,a 232 bp DNA sequence from the first exon of the P-ATPase gene,MgAPT2,was used as interfering fragment and inserted into interfering vector posi-tively and negatively. The interfering vector was then transformed into rice variety Nipponbare susceptible to rice blast.Rice blast resistance of transgenic plants and gene expression were checked. The results showed that the ex-pression level of the P-ATPase gene MgAPT2 from Magnaporthe grisea in transgenic rice decreased and blast-dis-ease resistance of transgenic rice increased. This study provided a new way for innovation of rice blast-disease resist-ance germplasm.

Key words: Rice, Blast resistance, Gene silencing

中图分类号: 

• S511