摘要： 叶绿体DNA标记是水稻分子生物学分析中的常用方法之一。但是水稻叶绿体DNA的提取过程复杂且耗时,为了简化分析步骤,本试验使用CTAB与SDS方法分别提取栽培水稻籼93-11和粳沈农265的叶、根、种子3种组织总DNA,选择3对叶绿体基因间隔区标记(psbA-trnH、petG-trnP和infA-rpl36)以及2对水稻叶绿体籼粳分类标记(ORF100和ORF29-TrnCGCA)进行PCR扩增。结果显示以它们种子、根为模板的扩增效果与叶片模板的扩增效果相一致,并与预期的结果相符合。最后对60份杂草稻种子总DNA进行叶绿体籼粳分类标记扩增分析,结果表明,杂草稻种子总DNA中扩增出目的片段同样能够进行有效的籼粳分类。这在一定程度上简化了水稻叶绿体标记的分析过程,加快分析速度。

关键词: 水稻, DNA提取, 叶绿体标记, PCR扩增

Abstract: "Chloroplast DNA marker is a common method of rice molecular analysis.However extract rice chloroplast DNA is a complex and time-consuming process.In order to simplify the steps of analyzing,this study use the CTAB and SDS methods to extract the total DNA(cultivation rice indica 93-11 and japonica SN265) from three different tissues of rice,including leafs roots and seeds,then amplify the total DNA templates with three pairs of chloroplast gene interval area markers(psbA-trnH,petG-trnP and infA-rpl36) and two pairs of chloroplast Indica-japonica classification markers(ORF100 and ORF29-TrnCGCA).The amplification results of roots and seeds are consistent with the leaf results and are also in expectation.And then use sixty weedy rice seed total DNA templates with chloroplast marks of Indica-japonica classification in PCR reaction.The results show these groups of chloroplast markers can amplify the target fragment from weedy rice seed total DNA and do Indica-japonica classification effectively.This conclusion can simplify the analysis process with rice chloroplast DNA markers and accelerate the analysis speed. "

Key words: Rice, DNA extraction, ChloroplastDNA marker, PCR amplification

中图分类号: 

• S511.03