华北农学报 ›› 2010, Vol. 25 ›› Issue (S1): 55-59. doi: 10.7668/hbnxb.20102.S1.014

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不同种源黄连木遗传多样性研究

王超1, 路丙社2, 白志英3, 王洁2, 冯献彬2   

  1. 1. 河北农业大学林学院, 河北 保定 071001;
    2. 河北农业大学园林与旅游学院, 河北 保定 071001;
    3. 河北农业大学生命科学学院, 河北 保定 071001
  • 收稿日期:2010-04-17 出版日期:2010-12-30
  • 通讯作者: 路丙社(1965-)男,河北新乐人,教授,博士,主要从事园林植物及经济林育种研究
  • 作者简介:王超(1982-),男,河北满城人,硕士,主要从事植物群体遗传多样性研究
  • 基金资助:
    河北省自然科学基金(2008000296)

Genetic Diversity of Different Geographical Populations of Pistacia chinesis Bunge

WANG Chao1, LU Bing-she2, BAI Zhi-ying3, WANG Jie2, FENG Xian-bin2   

  1. 1. College of Forestry,Agricultural University of Hebei, Baoding 071001, China;
    2. College of Landscape Architecture and Tourism,Agricultural University of Hebei, Baoding 071001, China;
    3. College of Life Science,Agricultural University of Hebei, Baoding 071001, China
  • Received:2010-04-17 Published:2010-12-30

摘要: 采用RAMP对我国不同地区的180份黄连木材料进行遗传多样性、种群内和种群间的遗传变异进行了研究。结果表明,13对引物组合所产生的115条DNA扩增片段中,有109条具有多态性,多态位点百分率PPB为94.78%;Shannon多样性指数I=0.5541,Nei's多样性指数H=0.38030,物种总基因多样性(Ht)为0.2963;种群内基因多样性(Hs)为0.3803,群体间基因分化系数(Gst)为0.2209,表明22.09%的变异存在于种群间,群体内的变异占总变异的77.91%;基因流(Nm)为1.7633,显示种群间的基因交流顺畅。

关键词: 黄连木, RAMP, 遗传多样性

Abstract: The genetic diversity of Pistacia chinesis Bunge. was analyzed using random amplified microsatellite polymorphism (RAMP ) markers. The results indicated that there was relatively high genetic diversitv in China. 115 bands were amplified from 6 populations in different habitats with 13 reliable primers,109 bands were polymorphic,The polymorphic percentage was 94. 78%; The Shannon information index I was 0. 554 1 and Nei's index H was 0. 380 30. The total gene diversity Ht was 0. 296 3,and the gene diversity within populations Hs was 0. 380 3.The Gst was 0. 220 9,77. 91% of genetic variation existed within populations while 22. 09% of genetic variation existed among populations,and the gene flow was 1. 763 3. There was a strong gene flow among the populations,So the genetic drift would not cause the genetic differentiation.

Key words: Pistacia chinesis Bunge, RAMP, Genetic diversity

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引用本文

王超, 路丙社, 白志英, 王洁, 冯献彬. 不同种源黄连木遗传多样性研究[J]. 华北农学报, 2010, 25(S1): 55-59. doi: 10.7668/hbnxb.20102.S1.014.

WANG Chao, LU Bing-she, BAI Zhi-ying, WANG Jie, FENG Xian-bin. Genetic Diversity of Different Geographical Populations of Pistacia chinesis Bunge[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 55-59. doi: 10.7668/hbnxb.20102.S1.014.

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