华北农学报 ›› 2010, Vol. 25 ›› Issue (5): 155-160. doi: 10.7668/hbnxb.2010.05.032

所属专题: 甜椒辣椒

• 论文 • 上一篇    下一篇

化感物质松香酸对辣椒种子萌发、幼苗生长及根际微生物的影响

周宝利1, 李燕1, 李东1, 刘娜1, 宁繁华2, 侯永侠3   

  1. 1. 沈阳农业大学, 园艺学院, 辽宁, 沈阳, 110161;
    2. 沈阳农业大学, 植物保护学院, 辽宁, 沈阳, 110161;
    3. 沈阳大学, 环境工程重点实验室, 辽宁, 沈阳, 110044
  • 收稿日期:2010-08-15 出版日期:2010-10-28
  • 作者简介:周宝利(1956-),男,辽宁绥中人,教授,博士,博士生导师,主要从事蔬菜栽培与生理生态研究.
  • 基金资助:
    国家"863"计划资助项目(2004AA247010)

Effects of Abietic Acid on Seed Germination,Seedling Growth and Microbial Population in Rhizosphere of Hot Pepper

ZHOU Baoli1, LI Yan1, LI Dong1, LIU Na1, NING Fanhua2, HOU Yongxia3   

  1. 1. College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China;

    2. College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China;

    3. Key Laboratory of Environmental Engineering, Shenyang University, Shenyang 110044, China
  • Received:2010-08-15 Published:2010-10-28

摘要: 为揭示辣椒连作障碍的机理,本试验探讨了松香酸对辣椒种子萌发、幼苗生长和根际微生物种群数量的影响.利用GC-MS分析的方法,研究了辣椒根系分泌物和连作土壤中的化感物质.结果表明:辣椒根系分泌物和连作土壤的主要化感物质为松香酸、十四酸甲酯、十八酸甲酯和(2-乙基-甲基苯)-N-(乙氧甲基)-2-氯代乙酰胺.并采用模拟的方法,研究了化感物质松香酸对辣椒种子萌发、幼苗生长发育及根际微生物的效应.对松香酸的生物测定结果显示,当浓度为0.05 g/L时,松香酸促进辣椒种子萌发和幼苗生长发育,其余浓度则表现为抑制作用,并且随着浓度的加大抑制作用增强,当浓度为0.25 g/L时抑制作用最大.同时外源松香酸使根际微生物总量和放线菌数量(A)减少,真菌(F)和细菌(B)的数量增加,B/F比值升高,而A/F比值降低.本试验结果说明松香酸对辣椒种子萌发、幼苗生长和根际微生物有较强的自毒作用.

关键词: 辣椒, 化感物质, 松香酸, 自毒作用

Abstract: The effects of abietic acid on seed germination,seedling growth and rhizosphere microbial populations of hot pepper were discussed. The allelochemicals in root exudates of hot pepper and monoculture soil were studied by GC-MS methods. The results showed that the main allelochemicals were identified as abietic acid,methyl tetradecanoate,octadecanoic acid,methyl ester,and acetamide 2-chloro-N-(ethoxymthyl) -N-(2-ethyl-6-methylphenyl,Allelopathic effects of abietic acid exudated by hot pepper root to seedling germination,growth and microbial population in rhizosphere soil were studied by using simulative method. The bioassay results indicated that abietic acid promoted seed germination and seedling growth of hot pepper at 0. 05 g /L. Whereas,inhibitory effects were found at other concentrations,and effects inhibitory rates were enhanced along with concentration increase. The maximum stimulative effect occurred at 0. 25 g /L. Compared with the control,abietic acid changed microbial community structure in rhizosphere of hot pepper,with decrease of actinomyces(A) and the ratios of A to F,increase of bacteria(B) ,fungi(F) ,and the ratios of B to F. The results indicated that abietic acid have strong autotoxic effects on seed germination,seedling growth and microbial population in rhizosphere soil of hot pepper.

Key words: Hot pepper, Allelopathy, Abietic acid, Autotoxicity

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引用本文

周宝利, 李燕, 李东, 刘娜, 宁繁华, 侯永侠. 化感物质松香酸对辣椒种子萌发、幼苗生长及根际微生物的影响[J]. 华北农学报, 2010, 25(5): 155-160. doi: 10.7668/hbnxb.2010.05.032.

ZHOU Baoli, LI Yan, LI Dong, LIU Na, NING Fanhua, HOU Yongxia. Effects of Abietic Acid on Seed Germination,Seedling Growth and Microbial Population in Rhizosphere of Hot Pepper[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 155-160. doi: 10.7668/hbnxb.2010.05.032.

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