辣椒Whirly基因家族的鉴定及表达分析

doi:10.7668/hbnxb.201750777

摘要/Abstract

摘要： 为了探索辣椒Whirly基因家族成员的功能和进化关系，通过生物信息学分析了其基因结构、保守基序、进化关系及表达模式，通过荧光定量PCR测定其在脱落酸、高温、低温、疫病等胁迫下的表达量。结果表明，从辣椒CM334中鉴定了2个Whirly基因家族成员，CaWHY1和CaWHY2。辣椒CaWHY1和CaWHY2同其他物种Whirly基因理化性质相比，差别不大，结构相似性高，CaWHY2基因和番茄的SlWHY2基因结构完全一样，在系统进化树中聚在一起。CaWHY1与SlWHY1聚在一起，但它们的基因结构不同，说明Whirly蛋白在进化过程中，结构保守。表达模式分析发现，CaWHY1和CaWHY2在辣椒CM334中均能表达，但在不同组织和果实发育时期的表达水平存在差异。CaWHY1和CaWHY2在不同胁迫处理下受到不同程度的诱导，其中CaWHY1明显受疫病的诱导，CaWHY2在低温处理和脱落酸胁迫下的表达量变化趋势相反。由此可知，Whirly基因家族在辣椒的生长发育中起调控作用，在各种逆境胁迫中也发挥一定作用。

关键词: 辣椒, Whirly基因家族, 全基因组鉴定, 胁迫, 表达分析

Abstract: To preliminarily explore the functions and evolutionary relations of Whirly gene family members, the bioinformatics was used to analyze the gene structure, conserved motif, evolutionary relationship and expression patterns, etc., and the fluorescence quantitative PCR was used to determine the expression levels under abscisic acid, high temperature, low temperature and epidemic stress. The results showed that two Whirly genes (CaWHY1 and CaWHY2) were identified from the pepper variety CM334. Compared with the physicochemical properties of other species, Whirly, the CaWHY1 and CaWHY2 have little difference in structure and similarity.The CaWHY2 gene had exactly the same genetic structure with the SlWHY2 gene of tomato, which were clustered together in phylogenetic tree. CaWHY1 and SlWHY1 were also clustered together, but their genetic structures were different, indicating that the structure of the Whirly protein is conserved during the evolution.The expression pattern analysis showed that both CaWHY1 and CaWHY2 could express in pepper CM334, but their expression levels were different in different tissues and at different fruit development stages. CaWHY1 and CaWHY2 were induced to different degrees under different stress treatments, among which CaWHY1 was obviously induced by epidemic diseases. The expression of CaWHY2 under low temperature and abscisic acid stress showed an opposite trend. Above results suggested that, Whirly gene family played a regulatory role in pepper growth and development, and also played a certaun role in various stress.

Key words: Pepper, Whirly gene family, Genomic identification, Stress, Expression analysis

中图分类号:

S641.03
Q78

吕艳艳, 魏小春, 赵艳艳, 原玉香, 王志勇, 杨双娟, 郑小兰, 姜俊, 李艳, 张梦园, 姚秋菊, 张强, 张晓伟. 辣椒Whirly基因家族的鉴定及表达分析[J]. 华北农学报, 2019, 34(2): 72-78. doi: 10.7668/hbnxb.201750777.

LÜ Yanyan, WEI Xiaochun, ZHAO Yanyan, YUAN Yuxiang, WANG Zhiyong, YANG Shuangjuan, ZHENG Xiaolan, JIANG Jun, LI Yan, ZHANG Mengyuan, YAO Qiuju, ZHANG Qiang, ZHANG Xiaowei. Identification and Expression Analysis of Whirly Gene Family in Capsicum annuum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 72-78. doi: 10.7668/hbnxb.201750777.

http://www.hbnxb.net/CN/Y2019/V34/I2/72

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