应用mtDNA Cytb基因全序列分析中国5个马鹿群体的遗传多样性和系统发育

doi:10.7668/hbnxb.2010.04.003

华北农学报 ›› 2010, Vol. 25 ›› Issue (4): 12-16. doi: 10.7668/hbnxb.2010.04.003

所属专题： 生物技术；

应用mtDNA Cytb基因全序列分析中国5个马鹿群体的遗传多样性和系统发育

张丽, 滚双宝, 雷天云, 刘丽霞, 秦大伟, 赵世峰

甘肃农业大学动物科技学院, 甘肃兰州 730070

收稿日期:2010-05-15 出版日期:2010-08-28
通讯作者: 滚双宝(1967- )，男，甘肃张掖人，教授，硕士生导师，主要从事动物遗传育种与繁殖、特种经济动物饲养研究。
作者简介:张丽(1980- ),女,宁夏石嘴山人,讲师,硕士,主要从事动物遗传育种与繁殖研究.
基金资助:
甘肃省科技攻关项目(2GS054-A41-002);甘肃省人事厅创新人才科研资助项目(036084)

Analysis of Genetic Diversity and Phylogeny of Five Chinese Subspecies of Wapiti Using Mitochondrial Cytb Complete Sequence

ZHANG Li, GUN Shuang-bao, LEI Tian-yun, LIU Li-xia, QIN Da-wei, ZHAO Shi-feng

Faculty of Animal Sci & Tech, Gansu Agricultural University, Lanzhou 730070, China

Received:2010-05-15 Published:2010-08-28

摘要/Abstract

摘要： 为了在分子水平上探讨马鹿的遗传多样性和系统发育,对我国个马鹿群体43头马鹿mtDNA Cytb基因全序列进行了分析.结果表明:个马鹿群体 mtDNA Cytb基因全序列中A、T、G、C含量没有明显差别;多态位点占分析位点的7.63%,天山马鹿和塔里木马鹿的单倍型比例分别为100%,0%,单倍型多样度(Hd)分别为(1.000±0.218),(0.700±0.096),核苷酸多样度(Pi)分别为0.003 33,0.00 44,平均核苷酸差异数值(K)分别为3.800,6.200,其遗传多样性较丰富.对13个马鹿单倍型序列的系统发生分析表明,我国马鹿有2个母系起源.从GenBank获得33个马鹿Cytb基因全序列与本研究的马鹿Cytb基因的单倍型进行网络关系分析,发现塔里木马鹿与西方马鹿群体具有较近的亲缘关系.

关键词: mtDNA, Cytb, 起源, 遗传多样性, 系统发育, 马鹿

Abstract: To examine molecular genetic diversity and phylogeny of wapiti，mitochondrial Cytb complete sequence have been analyzed in 43 wapiti from 5 Chinese populations. The results showed that content of A，T，G and C in mitochondrial Cytb complete sequence were not significantly different among 5 populations. Diverse loci took 7. 63% of analyzed loci. For Tianshan wapiti and Talimu wapiti respectively，haplotypes ratios were 100% and 50% ; haplotypes diversity (Hd) were (1. 000 ± 0. 218) and (0. 700 ± 0. 096) ; nucleotide diversity (Pi) were 0. 003 33 and 0. 005 44; average number of nucleotide difference (K) were 3. 800 and 6. 200. It is suggested that the 5 populations are highly genetic diverse. Analysis on haplotypes phylogeny of 13 wapiti showed that there were 2 maternal origins in China. Comparison between Cytb haplotypes of wapiti in this study to those of other 33 wapiti obtained from GenBank has been done using Network relation analysis. The results showed that the Talimu wapiti was phylogenetically close to wapiti populations in the western countries.

Key words: Mitochondrial DNA, Cytochrome b, Genetic diversity, Phylogenetic, Wapiti

中图分类号:

张丽, 滚双宝, 雷天云, 刘丽霞, 秦大伟, 赵世峰. 应用mtDNA Cytb基因全序列分析中国5个马鹿群体的遗传多样性和系统发育[J]. 华北农学报, 2010, 25(4): 12-16. doi: 10.7668/hbnxb.2010.04.003.

ZHANG Li, GUN Shuang-bao, LEI Tian-yun, LIU Li-xia, QIN Da-wei, ZHAO Shi-feng. Analysis of Genetic Diversity and Phylogeny of Five Chinese Subspecies of Wapiti Using Mitochondrial Cytb Complete Sequence[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 12-16. doi: 10.7668/hbnxb.2010.04.003.

http://www.hbnxb.net/CN/Y2010/V25/I4/12

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应用mtDNA Cytb基因全序列分析中国5个马鹿群体的遗传多样性和系统发育

Analysis of Genetic Diversity and Phylogeny of Five Chinese Subspecies of Wapiti Using Mitochondrial Cytb Complete Sequence

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应用mtDNA Cytb基因全序列分析中国5个马鹿群体的遗传多样性和系统发育

Analysis of Genetic Diversity and Phylogeny of Five Chinese Subspecies of Wapiti Using Mitochondrial Cytb Complete Sequence

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