华北农学报 ›› 2008, Vol. 23 ›› Issue (3): 28-30. doi: 10.7668/hbnxb.2008.03.008

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黄河中游地区粗山羊草高分子量谷蛋白亚基组成分析

张明, 苏亚蕊, 张大乐, 李锁平   

  1. 河南大学, 农业生物技术研究所, 河南, 开封 475001
  • 收稿日期:2007-10-12 出版日期:2008-06-28
  • 通讯作者: 李锁平(1963-),男,河南虞城人,教授,博士生导师,主要从事作物遗传育种研究。
  • 作者简介:张明(1968-),男,河南南阳人,硕士,主要从事植物分子细胞遗传学研究.
  • 基金资助:
    河南省高校杰出人才创新工程项目(HAIPURT,2007KYCX009)

The Analyse of Allelic Composition of the HMW Glutenin Subunits in Aegilops tauschii in Middle Reaches of the Yellow River

ZHANG Ming, SU Ya-rui, ZHANG Da-le, LI Suo-ping   

  1. Institute of Agricultural Biotechnology, Henan University, Kaifeng 475001, China
  • Received:2007-10-12 Published:2008-06-28

摘要: 为了解黄河中游地区粗山羊草的高分子量谷蛋白亚基(HMW-GS)遗传多样性,应用SDS-PAGE技术分析了56份粗山羊草Glu-Dt1位点的HMW-GS组成,检测到3种x-型亚基(5t,6.2t,null)和3种y-型亚基(10.5t,10.4t,10.3t)。其中6.2t类型是新的x-型亚基。供试粗山羊草共发现3种HMW-GS组合类型5t+10.5t,6.2t+10.4t,null+10.3t,它们的比例分别是91.07%,7.14%,1.79%。表明该地区粗山羊草的高分子量谷蛋白亚基有一定变异。

关键词: 粗山羊草, Glu-Dt1位点, 高分子量谷蛋白亚基, SDS-PAGE

Abstract: A Collection of 56 accessions of Aegllops tauschll was investigated for the allelic composition of the high molecular weight subunits at the Glu-Dt1 locus by SDS-PAGE analysis.Three x-type glutenin subunits(5t,6.2t,null)and Three y-type subunits(10.5t,10.4t,10.3t)were identified,respectively,according to their ascending mobility.Among them,subunit 6.2t was a novel x-type glutenin subunit.Three HMW-GS combinations(5t+10.5t,6.2t+10.4t,null+10.3t,)were found in these collection,of which their percent were 91.07%,7.14% and 1.79%.The results indicated that some genetic diversity were presented in the 56 accessions of Aegilops tauschii.

Key words: Aegllops tauschll, Qu-Dt 1 locus, HMW-GS, SDS-PAGE

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引用本文

张明, 苏亚蕊, 张大乐, 李锁平. 黄河中游地区粗山羊草高分子量谷蛋白亚基组成分析[J]. 华北农学报, 2008, 23(3): 28-30. doi: 10.7668/hbnxb.2008.03.008.

ZHANG Ming, SU Ya-rui, ZHANG Da-le, LI Suo-ping. The Analyse of Allelic Composition of the HMW Glutenin Subunits in Aegilops tauschii in Middle Reaches of the Yellow River[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 28-30. doi: 10.7668/hbnxb.2008.03.008.

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