人工合成小麦抗病性及其高分子量谷蛋白亚基的鉴定与评价

doi:10.7668/hbnxb.2010.06.023

摘要/Abstract

摘要： 为了发掘蕴藏于人工合成小麦中的优异种质资源,以138份人工合成小麦为材料,对其农艺性状、白粉病抗性、条锈病抗性和高分子量麦谷蛋白亚基进行了研究。根据农艺性状的鉴定结果,筛选出千粒重≥50 g、穗长≥12cm、穗粒数≥40粒和株高≤80 cm的种质分别为87份、24份、26份和19份。根据抗病性鉴定结果,筛选出抗白粉病种质27份,占总数的19.5%,其中免疫、高抗和中抗种质分别为21份、2份和4份;筛选出抗条锈病种质55份,占总数的39.9%,其中免疫、高抗和中抗材料分别为28份、12份和15份;其中10份人工合成兼抗白粉病和条锈病。SDS-PAGE分析表明,在Glu-A1位点上存在5种变异类型和1种新亚基;在Glu-B1位点上存在13种等位变异类型和5种新亚基,其中14+15、6+8、7+8分别占总数的21.7%,33.3%和29.7%;在Glu-D1位点上存在13种等位变异类型和4种新亚基,其中1.5+10、2+12、2+12.5、5+10分别占总数的21.7%,22.5%,16.7%,13.0%。结果表明,人工合成小麦在农艺性状、白粉病抗性、条锈病抗性和高分子量麦谷蛋白亚基方面存在丰富的遗传多样性,筛选的优异资源可以作为小麦遗传改良的基因资源。

关键词: 人工合成小麦, 农艺性状, 白粉病, 条锈病, 高分子量谷蛋白亚基

Abstract: Synthetic wheat is a important germplasm to transfer benefit gene(S)from alien species wheat to cornmort wheat(Tritium aestivum L.).Agronomic characters, powdery mildew resistance, the resistantance rust resistance, and high-molecular-weight glutenin subunit(HMW-GS)of 138 synthetic wheat were identificatied and evaluated in order to some benefit germplasm.The results of agronomic characters showed that the germplasm number of 1 000 grain weightI>50 g, spike length≥12 cm, stripe per spike≥40, and plant height≤80 cm were 87, 24, 26 and 19, respec— tively, number of grains identification results to powedery mildew showed that 27(19.5%)synthetic wheat accessions were resistant to powdery mildew and 21, 2 and 4 out of 27 accessions were immune, highly resistant and moderately resistant to powdery mildew, respectively.The resistant ante identification results to stripe rust showed that 55 (39.9%)synthetic wheat accessions were resistant to stripe rust and 28, 12 and 15 out of 55 accessions were immune. highly resistant and moderately resistant to stripe rust, respectively.10 synthetic wheat accessions were resistant to both powdery mildew and stripe rust.High-molecular—weight glutenin subunit(HMW—GS)were analyzed by SDSPAGE. Five alleles and one novel types of alleles were identified at the Glu—A1.Thirteen alleles and five novel types of alleles were identified at the Glu.Bl lOCUS and frequencies of subunit 14+15.6+8 and 7+8 were 21.7%。 33.3%, and 29.7%, respectively.Thirteen alleles and four novel types of alleles identified at the Glu.DI and frequen— cies of subunit 1.5+10, 2+12, 2+12.5 and 5+10 were 21.7%。22.5%, 16.7%and 13.0%respectively.The resuits showed that 138 synthetic wheat accessions at agronomic characters, powdery mildew resistance, stripe rust resist· anee, and HMW—GS have abundant genetic diversity.The benefit germplasm could be used to wheat improment.

Key words: Synthetic wheat, Agronomic characters, Powdery mildew, Stripe rust, High—molecular·weight glute&mdash, nin subunit(HMW—GS)

中图分类号:

S512.1

王亚娟, 王长有, 刘新伦, 张宏, 吉万全. 人工合成小麦抗病性及其高分子量谷蛋白亚基的鉴定与评价[J]. 华北农学报, 2010, 25(6): 122-127. doi: 10.7668/hbnxb.2010.06.023.

WANG Ya-juan, WANG Chang-you, LIU Xin-lun, ZHANG Hong, JI Wan-quan. Identification and Evaluation of Disease Resistance and HMW-GS of Synthetic Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 122-127. doi: 10.7668/hbnxb.2010.06.023.

http://www.hbnxb.net/CN/Y2010/V25/I6/122

参考文献

[1] 袁汉民,杨欣明,张富国,等.利用普通小麦与近缘属间的复合杂交创造小麦新种质[J].植物遗传资源学报,2004,5(4):320-323.
[2] 李振声,容珊,陈漱阳,等.小麦远缘杂交[M].北京:科学出版社,1985:141-145.
[3] 董玉琛,郑殿升.中国小麦遗传资源[M].北京:中国农业出版社,2006:195-204,224-227.
[4] 胡含,王恒.植物细胞工程与育种[M].北京:北京工业大学出版社,1990:125-131.
[5] 王世红,周宽基,冯毓琴,等.硬粒小麦-节节麦人工合成小麦的鉴定和利用[J].甘肃农业科技,2006,(7):12-15.
[6] 许树军,董玉琛,周荣华,等.小麦与山羊草双二倍体抗性的研究与利用[J].作物学报,1990,16(2):106-111.
[7] 胡英考,辛志勇,陈孝,等.硬粒小麦--粗山羊草双二倍体白粉病抗性的遗传分析与基因推导[J].遗传学报,2001,28(2):152-157.
[8] Liu Z Y,Sun Q X,Ni Z F,et al.Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild ernmer[J].Euphytica,2002,123:21-29.
[9] Shi A N,Leath S,Murphy J P.A major gene for powdery midew resistance transferred to common wheat from einkorn wheat[J].Phytopathology,1998,88:144-147.
[10] 倪中福,张荣,梁荣奇,等.从CIMMYT引进的人工合成六倍体小麦D染色体组微卫星分子标记的遗传差异[J].遗传学报,2002,29(6):542-548.
[11] 杨林,李霞,刘登才,等.人工合成多倍体小麦及其供体种的高分子蛋白亚基组成研究[J].农业生物技术,2006,22(4):44-47.
[12] 王丽敏,林志珊,张增艳,等.人工合成小麦高分子量谷蛋白亚基组成分析及新亚基的发现[J].西南农业学报,2006,19(1):1-4.
[13] Jiang J M,Friehe B,Gill B S.Rcent advances in alien gene transfer in wheat[J].Euphytica,1994,73:199-212.
[14] 王亚娟,王耀勇,张德华,等.节节麦农艺性状及高分子量谷蛋白亚基遗传多样性研究[J].西北植物学报,2007,27(10):1967-1972.
[15] Payne P I,Lawrence G J.Catalogue of alleles for the complex gene loci,,Glu-A1,Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat[J].Cereal Research Communication,1983,11:29-35.
[16] Lawrence G J,Payne P I.Detection by gel electrophoresis of oligomers formed bythe association of high-molecular-weight glutenin protein subunits of wheat endosperm[J].Exp Bot,1983,34:254-267.
[17] Halford N Q,Fiele J M,Blair H,et al.Analysis of HMW glutenin subunits encoded chromosome 1A of bread wheat(Triticum aestivum L.)indicates quantitative effects on grain quality[J].Theor Appl Genet,1992,83:373-378.
[18] 颜国荣,白玉亭,王威,等.小麦品种资源农艺性状鉴定与评价[J].新疆农业科学,2006,43(S1):88-89.
[19] Del Blanco I A,Rajaram S,Kronstad W E.Agronomic potential of synthetic hexaploid wheat-derived populations[J].Crop Science,2001,41:670-676.
[20] 杨武云,颜济,杨俊良,等.硬粒小麦-节节麦人工合成种Decoy1/Aegilops tauschii510抗条锈性状(条中30,条中31)的遗传分析[J].西南农业学报,1999,12(2):38-41.
[21] 张颙,杨武云,郑有良,等.源于硬粒小麦-节节麦人工合成种的小麦新品种川麦38抗条锈性及遗传分析[J].西南农业学报.2006,19(1):14-18.
[22] 张颙,杨武云,胡晓蓉,等.源于硬粒小麦-节节麦人工合成种的高产抗病小麦新品种川麦42主要农艺性状分析[J].西南农业学报,2004,17(2):141-146.
[23] 张义荣,倪中福,梁荣奇,等.从CIMMYT引进的人工合成六倍体小麦高分子量谷蛋白亚基组成分析[J].中国农业大学学报,2001,6(3):38-43.
[24] Lagudaah E S,Hilalloran C M.Phylogenetic relationships of Triticum tavschii D genome donor to hexaploid wheat:Ⅰ.Variation in HMW svbunits of glvtenin and gliadins[J].Theor Appl Genet,1988,75:592-598.
[25] William M D H M,Penn R J,Mujeed-Kazi.A.Seed protein and isozyme variation in Triticum tauschii caegilops squarrosas[J].Theor Appl Genet,1993,87:257-263.

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