华北农学报 ›› 2015, Vol. 30 ›› Issue (S1): 103-106. doi: 10.7668/hbnxb.2015.S1.019

所属专题: 小麦

• 论文 • 上一篇    下一篇

长江中下游麦区小麦种质高分子量谷蛋白亚基组成分析

杨丹, 姚金保, 杨学明, 周淼平, 马鸿翔   

  1. 江苏省农业科学院 江苏省农业生物学重点实验室, 江苏 南京 210014
  • 收稿日期:2015-09-09 出版日期:2015-12-28
  • 通讯作者: 姚金保(1962-),男,江苏常熟人,研究员,硕士,主要从事小麦遗传育种研究.
  • 作者简介:杨丹(1980-),女,黑龙江齐齐哈尔人,副研究员,博士,主要从事小麦品质分析研究.
  • 基金资助:
    农业部小麦现代农业产业技术体系项目(CARS-03);江苏省农业科技自主创新资金项目(CX122026);江苏省科技支撑项目(BE2014351)

Composition of HMW-GS in Wheat Germplasm from the Middle and Low Reaches of Yangtze River

YANG Dan, YAO Jin-bao, YANG Xue-ming, ZHOU Miao-ping, MA Hong-xiang   

  1. Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2015-09-09 Published:2015-12-28

摘要: 为了解长江中下游麦区小麦种质的遗传多样性,挖掘可供利用的优异高分子量谷蛋白亚基(HMW-GS),为品种品质改良提供基础材料.利用聚丙烯酰胺凝胶电泳(SDS-PAGE)方法,分析了319份小麦种质的HMW-GS组成.结果表明: Glu-A1Glu-B1Glu-D1 位点分别具有3,7,4种不同的亚基类型.其中,亚基N、7+8和2+12在各自位点上出现的频率最高,分别达到了49.21%,54.55%,68.65%.亚基组成类型共有24种,1/7+8/2+12为出现频率最高的组合形式,频率为25.39%;其次为N/7+8/2+12、N/7+9/2+12和N/7+9/5+10,出现频率分别为17.24%,10.97%,10.34%;其他亚基组合类型出现的频率较低.同时,筛选出了一些含有2*、17+18、7+8、14+15和5+10等优质亚基类型的材料.

关键词: 长江中下游, 小麦种质, 高分子量谷蛋白亚基

Abstract: In order to understand the genetic diversity of wheat germplasm from the middle and low reaches of Yangtze River in China and provide the basic materials for quality improvement of wheat variety,319 accessions of wheat germplasm from the middle and low reaches of Yangtze River were analyzed with SDS-PAGE to reveal the allele variant of high molecular weight gluten subunits(HWM-GS).The results showed that there were 14 alleles on Glu-1 loci,3 alleles on Glu-A1 loci,7 alleles on Glu-B1 loci,4 alleles on Glu-D1 loci.The frequencies of N,7+8,2+12 alleles were the highest on Glu-A1, Glu-B1 and Glu-D1 loci,and were 49.21%,54.55%,68.65%,respectively.A total of 24 HMW-GS combination forms were identified in these materials,among which combination form(1/7+8/2+12) was the most frequent(25.39%),followed by(N/7+8/2+12)(17.24%),(N/7+9/2+12)(10.97%), (N/7+9/5+10)(10.34%),and the rest were rare subunit combinations,with frequency of 0.31%-6.90%.The materials containing elite subunits,such as 2*,17+18,7+8,14+15,5+10,were identified,and could be used as the origin of elite gene.

Key words: The Yangtze River Region, Wheat germplasm, HMW-GS

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引用本文

杨丹, 姚金保, 杨学明, 周淼平, 马鸿翔. 长江中下游麦区小麦种质高分子量谷蛋白亚基组成分析[J]. 华北农学报, 2015, 30(S1): 103-106. doi: 10.7668/hbnxb.2015.S1.019.

YANG Dan, YAO Jin-bao, YANG Xue-ming, ZHOU Miao-ping, MA Hong-xiang. Composition of HMW-GS in Wheat Germplasm from the Middle and Low Reaches of Yangtze River[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 103-106. doi: 10.7668/hbnxb.2015.S1.019.

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