摘要： 利用抑制差减杂交(Suppression subtractive hybridization，SSH)技术分离玉米低锌与正常供锌条件下表达有差异的cDNA片段。以低锌培养的玉米幼苗为试验方，正常供锌的玉米幼苗为对照方，建立正向差减文库；以正常供锌的玉米幼苗为试验方，以低锌诱导培养的玉米幼苗为对照方，建立反向差减文库。正、反向文库分别收集低锌和正常供锌条件下特异表达的cDNA片段。在2个文库中，各随机挑选30个克隆进行测序。序列分析表明，在正向差减文库中，分离得到的基因多与抗病防御、信号转导、细胞生长与分裂等方面相关；在反向差减文库中，分离得到的基因多与细胞组织发生及代谢等方面有关。

关键词: 抑制差减杂交, 玉米, 低锌

Abstract: Suppression subtractive hybridization (SSH) was performed to isolate the differentially expressed cDNA fragments under low-zinc and zinc-sufficiency conditions. In the forward subtracted library, the cDNA of maize seedlings treated with low-zinc was used as tester and the cDNA of maize seedlings treated with zinc-sufficiency was used as driver. In the reverse subtracted library, the cDNA of maize seedlings treated with zinc-sufficiency was used as tester and the cDNA of maize seedlings treated with low-zinc was used as driver. The cDNA fragments differentially expressed under low-zinc and zinc-sufficiency conditions were collected in the forward and reverse subtracted library, respectively. Thirty clones were randomly picked to sequence in each library. The sequencing results shows: the function of isolated genes in the forward subtracted library mostly relates to disease resistance/defense, signal transduction, cell growth/division and so on. While the function of isolated genes in the reverse subtracted library mostly relates to cell/tissue generation, metabolism and so on.

Key words: Suppression subtractive hybridization, Maize, Low-zinc

中图分类号: 

• Q786