烟粉虱取食ToCV侵染番茄对其解毒酶和保护酶基因表达的影响

doi:10.7668/hbnxb.20192207

华北农学报 ›› 2021, Vol. 36 ›› Issue (4): 172-183. doi: 10.7668/hbnxb.20192207

所属专题： 番茄；生物技术；

• 资源环境·植物保护 • 上一篇下一篇

烟粉虱取食ToCV侵染番茄对其解毒酶和保护酶基因表达的影响

代惠洁¹, 程琳⁴, 曹慧², 竺晓平³, 赵静²

1. 潍坊科技学院, 山东省高校设施园艺重点实验室, 山东寿光 262700;
2. 潍坊学院生物与农业工程学院, 山东省生物化学与分子生物学高校重点实验室, 山东潍坊 261061;
3. 山东农业大学植物保护学院, 山东省蔬菜病虫生物学重点实验室, 山东泰安 271018;
4. 山东寿光蔬菜种业集团有限公司, 山东省设施蔬菜分子育种省级重点实验室, 山东寿光 262700

收稿日期:2021-04-18 出版日期:2021-08-28
通讯作者: 赵静(1983-),女,山东肥城人,副教授,博士,主要从事蔬菜害虫综合治理研究。
作者简介:代惠洁(1979-),女,山东潍坊人,副教授,博士,主要从事蔬菜害虫综合治理研究。
基金资助:
山东省农业重大应用技术创新项目（SD2019ZZ004）；山东省重点研发计划（2019GSF109118；2019JZZY010707）；博士科研启动基金（2019BS10）；潍坊市科技发展计划（2019GX074）

The Influence of Bemisia tabaci MED on Detoxification and Protective Enzymes after Feeding on ToCV-infected Tomato

DAI Huijie¹, CHENG Lin⁴, CAO Hui², ZHU Xiaoping³, ZHAO Jing²

1. Key Laboratory of Facility Horticulture of Shandong Universities, Weifang University of Science and Technology, Shouguang 262700, China;
2. Key Laboratory of Biochemistry and Molecular Biology of Shandong Universities, College of Biological and Agricultural Engineering, Weifang University, Weifang 261061, China;
3. Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Pests, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China;
4. Shandong Provincial Key Laboratory of Facility Vegetable Molecular Breeding, Shandong Shouguang Vegetable Seed Industry Group, Shouguang 262700, China

Received:2021-04-18 Published:2021-08-28

摘要/Abstract

摘要： 为明确烟粉虱取食ToCV侵染番茄后其生理防御反应相关基因的变化，通过转录组测序和实时荧光定量PCR技术研究烟粉虱MED隐种取食ToCV侵染番茄和健康番茄6，12，24，48 h，其体内解毒酶、保护酶系统以及基因功能、代谢功能的变化。结果表明，烟粉虱MED隐种取食ToCV侵染番茄不同时间，其体内解毒酶细胞色素P450酶（P450）、谷胱甘肽S-转移酶（GST）、羧酸酯酶（CarE）和保护酶超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）转录水平均发生不同程度的变化；除CAT外，P450、GST、CarE、SOD和POD表达量总体趋于上调，其中发挥主要作用的是P450和SOD。通过对差异表达基因进行GO注释和KEGG功能分析，发现烟粉虱MED隐种取食ToCV侵染番茄12 h，其差异基因的生物学功能和代谢途径与其他时间点不同，该时间点下烟粉虱主要通过溶酶体实现蛋白质分解，而其他时间点下烟粉虱则通过核糖体逐步实现氨基酸到蛋白质的生物合成。综上，烟粉虱取食ToCV侵染番茄可能引起了其解毒酶、保护酶基因表达的改变，并通过溶酶体途径调控自身免疫应对ToCV入侵；其中，12 h可能是烟粉虱与ToCV互作的关键时间点。研究结果有助于进一步阐明烟粉虱取食ToCV获毒后的防御机制，丰富烟粉虱-植物病毒-寄主植物间的互作理论，为田间ToCV的有效防控提供科学依据。

关键词: 烟粉虱MED隐种, 转录组, 番茄褪绿病毒, 解毒酶, 保护酶

Abstract: To clarify the defense responses of Bemisia tabaci after feeding on ToCV-infected tomato, RNA-Seq analysis and qRT-PCR were performed to explore changes that occurred with the internal detoxification and protective enzyme systems as well as with the gene expression and metabolic functions 6, 12, 24, 48 hours respectively after B. tabaci MED fed on both ToCV-infected and uninfected tomato plants. The results showed that the transcription level of detoxification enzymes P450, GST, CarE and protective enzymes SOD, POD, CAT made different changes when B. tabaci MED feeding on ToCV-infected tomato at different times. The genes expression levels of P450, GST, CarE, SOD and POD tended to be up-regulated with the exception of CAT, among which P450 and SOD played the most major roles. Through GO annotation and KEGG function analysis of DEGs, it was found that biological function and metabolic pathways of DEGs of B. tabaci MED after feeding on ToCV-infected tomato for 12 h were different from other time points. After feeding for 12 h, B. tabaci MED mainly decomposed protein through lysosomes, while biosynthesis of amino acids to proteins were carried out through ribosomes at other times. In conclusion, B. tabaci that feeding on ToCV-infected tomato might have caused changes on the gene expression of detoxification and protective enzymes, and regulated the autoimmune response to ToCV invasion through lysosomal pathway. Among them, 12 hours feeding period might be the critical point for B. tabaci to interact with ToCV. The results here are helpful to further clarify the defense mechanism of B. tabaci toward ToCV infections, enrich the interaction theory of B. tabaci -ToCV-host, and provide a scientific basis for effective control of ToCV in the field.

Key words: Bemisia tabaci MED, Transcriptome, Tomato chlorosis virus, Detoxification enzymes, Protective enzymes

中图分类号:

S436.4

代惠洁, 程琳, 曹慧, 竺晓平, 赵静. 烟粉虱取食ToCV侵染番茄对其解毒酶和保护酶基因表达的影响[J]. 华北农学报, 2021, 36(4): 172-183. doi: 10.7668/hbnxb.20192207.

DAI Huijie, CHENG Lin, CAO Hui, ZHU Xiaoping, ZHAO Jing. The Influence of Bemisia tabaci MED on Detoxification and Protective Enzymes after Feeding on ToCV-infected Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 172-183. doi: 10.7668/hbnxb.20192207.

http://www.hbnxb.net/CN/Y2021/V36/I4/172

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烟粉虱取食ToCV侵染番茄对其解毒酶和保护酶基因表达的影响

The Influence of Bemisia tabaci MED on Detoxification and Protective Enzymes after Feeding on ToCV-infected Tomato

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