<i>N</i>-3-羰基十四烷酰基高丝氨酸内酯诱导 番茄对灰霉病抗性机制初探

doi:10.7668/hbnxb.20190995

摘要/Abstract

摘要： 为了探究细菌群体感应（QS）信号分子N-酰基高丝氨酸内酯（AHLs）在植物上诱导对于真菌病害的抵抗能力。以番茄为试验材料，以灰霉为病原指示菌，通过不同侧链长度和侧链修饰的AHLs信号分子预处理后接种病原菌，发现长链信号分子N-3-羰基十四烷酰基高丝氨酸内酯（3OC14-HSL）对灰霉有最佳的抗性效果。实时荧光定量PCR检测抗病基因发现，与未处理对照组相比，信号分子3OC14-HSL预处理能显著诱导接种灰霉病后的番茄体内与茉莉酸（JA）信号通路相应基因PI1、PI2上调；而与水杨酸信号（SA）通路相关的NPR1基因显著下调，PR1基因则没有显著变化。进一步通过DAB染色、过氧化氢含量测定以及过氧化物酶（POD）和超氧化物歧化酶（SOD）活性检测发现，3OC14-HSL预处理相比未处理对照组使得番茄产生大量活性氧，且可以保持较高的POD、SOD活性。这些结果表明，长链信号分子N-3-羰基十四烷酰基高丝氨酸内酯可以诱导番茄对灰霉的抗性，而且该抗性效果可能与茉莉酸信号路径相关。

关键词: 番茄, N-酰基高丝氨酸内酯, 灰霉, 抗性

Abstract: In order to determine the bacterial quorum sensing (QS) signaling molecule, N -acyl-homoserine lactones (AHLs) induce resistance to fungal diseases in plants. Tomato was used as the test material, Botrytis cinerea was used as the pathogen indicator bacteria, and AHLs signal molecules with different side chain length and side chain modification were pretreated to inoculate the pathogen bacteria, and the long chain signal molecule N-3-oxo-tetradecanoyl was found. 3OC14-HSL had the best resistance to gray mold. Real-time quantitative PCR detection of disease resistance genes revealed that the signal molecule 3OC14-HSL pretreatment significantly induced up-regulation of the genes PI1 and PI2 in the tomato and jasmonic acid (JA) signaling pathway compared with the untreated control group; However, the NPR1 gene related to the salicylic acid signaling (SA) pathway was significantly down-regulated, but the PR1 gene was not significantly changed. Further DAB staining, determination of hydrogen peroxide content, and detection of peroxidase (POD) and superoxide dismutase(SOD)enzyme activities revealed that 3OC14-HSL pretreatment caused a large amount of reactive oxygen species in tomatoes compared to the untreated control group. 3OC14-HSL also could maintain high POD, SOD activity. These results indicated that the long-chain signaling molecule N- 3-oxo-tetradecanoyl-homoserine(3OC14-HSL) could induce resistance to gray mold of tomato, and the resistance effect might be related to the jasmonic acid signal pathway.

Key words: Tomato, N-acyl-homoserine lactone, Botrytis cinerea, Resistance

中图分类号:

S432.4

杨向云, 刘方, 赵芊, 宋水山, 张利平. N-3-羰基十四烷酰基高丝氨酸内酯诱导番茄对灰霉病抗性机制初探[J]. 华北农学报, 2020, 35(4): 169-176. doi: 10.7668/hbnxb.20190995.

YANG Xiangyun, LIU Fang, ZHAO Qian, SONG Shuishan, ZHANG Liping. The Exploration of the Effect of N -3-oxo-tetradecanoyl Homoserine Lactone on the Resistance to Botrytis cinerea on Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 169-176. doi: 10.7668/hbnxb.20190995.

http://www.hbnxb.net/CN/Y2020/V35/I4/169

参考文献

[1] Bassler B L,Wright M,Showalter R E,Silverman M R.Intercellular signalling in Vibrio harveyi:sequence and function of genes regulating expression of luminescence[J]. Mol Microbiol,1993, 9(4):773-786.doi:10.1111/j.1365-2958.1993.tb01737.x.
[2] Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria:the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. J Bacteriol, 1994,176(2):269-275.doi:10.1128/jb.176.2.269-275.1994.
[3] Zhao Q, Zhang C, Jia Z H, Huang Y L, Li H L, Song S S. Involvement of calmodulin in regulation of primary root elongation by N -3-oxo-hexanoyl homoserine lactone in Arabidopsis thaliana[J]. Front Plant Sci,2015, 5:807.doi:10.3389/fpls.2014.00807.
[4] Zhao Q, Li M, Jia Z H, Liu F, Ma H, Huang Y L, Song S S.AtMYB44 positively regulates the enhanced elongation of primary roots induced by N -3-oxo-hexanoyl homoserine lactone in Arabidopsis thaliana[J]. Mol Plant Microbe Interact,2016, 29(10):774-785.doi:10.1094/MPMI-03-16-0063-R.
[5] Shenk S T, Schikora A. AHL-priming functions via oxylipin and salicylic acid[J]. Front Plant Sci,2015, 5:784.doi:10.3389/fpls.2014.00784.
[6] 赵芊, 宋水山. N -酰基高丝氨酸内酯调控植物生长发育的研究进展[J]. 植物生理学通讯,2010, 46(10):980-984.doi:10.13592/j.cnki.ppj.2010.10.005. Zhao Q,Song S S. Advances in the regulation of plant growth and development by N -acyl-homoserine lactones[J]. Plant Physiology Newsletter,2010,46(10):980-984.
[7] 赵芊, 贾振华, 宋水山. 细菌信号分子 N -酰基高丝氨酸内酯调控植物抗性的研究进展[J]. 植物生理学报, 2014,50(2):143-149.doi:10.13592/j.cnki.ppj.2014.02.003. Zhao Q,Jia Z H,Song S S. Advances in regulation of plant resistance by N-acyl-homoserine lactones,the bacterial quorum-sensing signals[J]. Plant Physiology Journal, 2014,50(2):143-149.
[8] Oridi M E,Bouarab K. Plant signalling components EDS1 and SGT1 enhance disease caused by the necrotrophic pathogen Botrytis cinerea[J]. New Phytologist,2007,175(1):131-139.doi:10.1111/j.1469-8137.2007.02086.x.
[9] Abramovitch R B,Martin G B. AvrPtoB:a bacterial type Ⅲ effector that both elicits and suppresses programmed cell death associated with plant immunity[J]. Fems Microbiology Letters,2005,245(1):1-8.doi:10.1016/j.femsle.2005.02.025.
[10] Bouarab K,Melton R,Peart J,Baulcombe D,Osboutn A.A saponin-detoxifying enzyme mediates suppression of plant defences[J] .Nature, 2002,418(6900):889-892.doi:10.1038/nature00950.
[11] Chisholm S T,Coaker G,Day B,Staskawicz B J. Host-microbe interactions:shaping the evolution of the plant immune response[J]. Cell, 2006,124(4):803-814.doi:10.1016/j.cell.2006.02.008.
[12] Hann D R,Gimenez-Ibanez S,Rathjen J P.Bacterial virulence effectors and their activities[J]. Current Option in Plant Biology,2010,13(4):388-393.doi:10.1016/j.pbi.2010.04.003.
[13] Janjusevic R,Abramovitch R B,Martin G B,Stebbins C E.A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase[J]. Science,2006,311(5758):222-226.doi:10.1126/science.1120131.
[14] Nomura K, DebRoy S, Lee Y H, Pumplin N, Jones J, He S Y.A bacterial virulence protein supresses host innate immunity to cause plant disease[J]. Science,2006,313(5784):220-223.doi:10.1126/science.1129523.
[15] Rigano L A, Payette C, Brouillard G, Marano M R, Abramowicz L,Torres P S,Yun M, Castagnaro A P, Oirdi E M, Dufour V, Malamud F, Dow J M, Bouarab K,Vojnov A A.Bacterial cyclic β-(1,2)-glucan acts in systemic suppression of plant immune responses[J]. The Plant Cell, 2007,19(6):2077-2089.doi:10.1105/tpc.106.047944.
[16] Yun M H,Torres P S, Oirdi M E, Rigano L A,Gonzalez-Lamothe R, Marano M R,Castagnaro A P,Dankert M A,Bouarab K,Vojnov A A. Xanthan induces plant susceptibility by suppressing callose deposition[J]. Plant Physiology,2006,141(1):178-187.doi:10.1104/pp.105.074542.
[17] Mou Z L, Fan W H, Dong X N. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes[J]. Cell,2003,113(7):935-944.doi:10.1016/s0092-8674(03)00429-x.
[18] 邹庆道,张子君,徐明,李海涛.番茄抗灰霉病材料叶、茎和果实抗性的相关性研究[J].园艺学报,2009,36(S1):1972. Zou Q D, Zhang Z J, Xu M, Li H T. Studies on the correlation of leaf, stem and fruit resistance of tomato to gray mold resistance materials[J]. Acta Horticulturae Sinica, 2009, 36 (S1):1972.
[19] 田龙,淡昭菊,关迎池.大棚番茄灰霉病流行规律观察试验[J].湖北植保,2017(2):9-11.doi:10.3969/j.issn.1005-6114.2017.02.005. Tian L, Dan Z J, Guan Y C. Observation and experiment on the epidemic law of tomato gray mold in greenhouses[J]. Hubei Plant Protection, 2017(2):9-11.
[20] Thordal-Christensen H, Zhang Z G, Wei Y D, Collinge D B. Subcellular localization of H₂O₂ in plants. H₂O₂ accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction[J]. The Plant Journal,1997,11(6):1187-1194.doi:10.1046/j.1365-313X.1997.11061187.x.
[21] Lamb C, Dixon R A. The oxidative burst in plant disease resistance[J]. Annu Rev Plant Mol Biol,1997,48:251-275.doi:10.1146/annurev.arplant.48.1.251.
[22] Wojtaszek P.Oxidative burst:an early plant response to pathogen infection[J]. Biochem,1997,322(3):681-692.doi:10.1042/bj3220681.
[23] Thoma I, Loeffler C, Sinha A K, Gupta M, Krischke M, Steffan B,Roitsch T, Mueller M J. Cyclopentenone isoprostanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in plants[J]. The Plant Journal,2003,34(3):363-375.doi:10.1046/j.1365-313x.2003.01730.x.
[24] Hartmann A, Schenk S T, Riedel T, Schröder P, Schikora A.The response of plants toward N -acyl homoserine lactones of quorum-sensing-active bacteria in the rhizosphere[J]. Molecular Microbial Ecology of the Rizosphere 1 & 2,2013,73:77-783.doi:10.1002/9781118297674.ch73.
[25] Schikora A, Schenk S T, Stein E, Molitor A, Zuccaro A, Kogel K H.N-acyl-homoserine lactone confers resistance towards biotrophic and hemibiotrophic pathogens via altered activation of AtMPK6[J]. Plant Physiol, 2011,157(3):1407-1418.doi:10.1104/pp.111.180604.
[26] Schuhegger R, Ihring A, Gantner S, Bahnweg G, Knappe C,Vogg G, Hutzler P, Schmid M, Breusegem F V, Eberl L, Hartmann A, Langebartels C.Induction of systemic resistance in tomato by N -acyl-L-homoserine lactone-producing rhizosphere bacteria[J].Plant Cell Environ, 2006, 29(5):909-918.doi:10.1111/j.1365-3040.2005.01471.x.
[27] Zarkani A A, Stein E, Röhrich C R, Schikora M, Evguenieva-Hackenberg E,Degenkolb T, Vilcinskas A, Klug G, Kogel K H, Schikora A. Homoserine lactones influence the reaction of plants to rhizobia[J]. Int J Mol Sci, 2013,14(8):17122-17146.doi:10.3390/ijms140817122.
[28] Hernández-Reyes C, Schenk S T, Neumann C, Kogel K H,Schikora A.N-acyl-homoserine lactones-producing bacteria protect plants against plant and human pathogens[J]. Microb Biotechnol, 2014,7(6):580-588.doi:10.1111/1751-7915.12177.
[29] Hu Z J,Shao S J,Zheng C F,Sun Z H,Shi J Y,Yu J Q,Qi Z Y,Shi K.Induction of systemic resistance in tomato against Botrytis cinerea by N -decanoyl-homoserine lactone via jasmonic acid signaling[J]. Planta,2018, 247(5):1217-1227.doi:10.1007/s00425-018-2860-7.
[30] Liu F, Zhao Q, Jia Z H, Song C, Huang Y L, Ma H, Song S S. N -3-oxo-octanoyl-homoserine lactone-mediated priming of resistance to Pseudomonas syringae requires the salicylic acid signaling pathway in Arabidopsis thaliana[J]. BMC Plant Biology,2020,20:38.doi:10.1186/s12870-019-2228-6.
[31] Birkennbihl R P, Diezel C, Somssich I E. Arabidopsis WRKY33 is a key transcriptional regulator of hormonal and metabolic responses toward Botrytis cinerea infection[J]. Plant Physiol,2012,159(1):266-285.doi:10.1104/pp.111.192641.
[32] Zabala M D T, Zhai B, Jayaraman S, Eleftheriadou G, Winsbury R,Yang R,Truman W,Tang S J, Smirnoff N,Grant M.Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection[J]. New Phytol,2016,209(3):1120-1134.doi:10.1111/nph.13683.
[33] Zhu Z Q, An F Y, Feng Y, Li P P, Xue L, A M, Jiang Z Q, Kim J M, To T K, Li W, Zhang X Y, Yu Q, Dong Z,Chen W Q, Seki M, Zhou J M, Guo H W. Derepression of ethylene-stabilized transcription factors(EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis[J]. Proc Natl Acad Sci,2011,108(30):12539-12544. doi:10.1073/pnas.1103959108.
[34] Zhang S, Li X, Sun Z H, Sun S J, Hu L F,Ye M, Zhou Y H, Xia X J, Yu J Q, Shi K.Antagonism between phytohormone signaling underlies the variation in disease susceptibility of tomato plants under elevated CO₂[J]. Journal of Experimental Botany,2015,66(7):1951-1963.doi:10.1093/jxb/eru538.
[35] Ferrari S, Galletti R, Denoux C, De Lorenzo G, Ausubel F M, Dewdney J.Resistance to Botrytis cinerea induced in Arabidopsis by elicitors is independent of salicylic acid,ethylene,or jasmonate signaling but requires PHYTOALEXIN DEFICIENT3[J]. Plant Physiol,2007,144(1):367-379.doi:10.1104/pp.107.095596.
[36] Rowe H C, Walley J W, Corwin J, Chan E K F, Dehesh K, Kliebenstein D J.Deficiencies in jasmonate-mediated plant defense reveal quantitative variation in Botrytis cinerea pathogenesis[J]. PLoS Pathog,2010,6(4):e1000861.doi:10.1371/journal.ppat.1000861.
[37] Rehman S,Aziz E,Akhtar W,Ilyas M,Mahmood T.Structural and functional characteristics of plant protecinase inhibitor-Ⅱ(PI-Ⅱ)familiy[J]. Biotechnol Lett,2017,39(5):647-666.doi:10.1007/s10529-017-2298-1.

选择文件类型/文献管理软件名称

选择包含的内容

N-3-羰基十四烷酰基高丝氨酸内酯诱导番茄对灰霉病抗性机制初探

The Exploration of the Effect of N -3-oxo-tetradecanoyl Homoserine Lactone on the Resistance to Botrytis cinerea on Tomato

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王亚, 王越涛, 申关望, 王付华, 王生轩, 白涛, 尹海庆. *聚合R基因Pigm和非R基因bsr-d1改良水稻稻瘟病抗性*[J]. 华北农学报, 2022, 37(5): 157-165.
[2]	李万星, 李丹, 李小霞, 曹晋军, 靳鲲鹏, 韩文清, 苏秀敏, 王佼, 黄学芳, 刘永忠. 不同轮作模式对旱地番茄品质、产量及土壤真菌多样性的影响[J]. 华北农学报, 2022, 37(4): 82-89.
[3]	贾志强, 许云玉, 高雪, 陶宏征, 陈增敏, 刘雅婷, 李永忠. *辣椒CaWRKY30转录因子的克隆、亚细胞定位及番茄斑萎病毒侵染下的表达分析*[J]. 华北农学报, 2022, 37(3): 186-192.
[4]	史建磊, 熊自立, 苏世闻, 王克磊, 宰文珊. 基于RNA-seq的番茄青枯病响应基因鉴定与表达分析[J]. 华北农学报, 2022, 37(2): 171-182.
[5]	高素红, 葛长虹, 周国娜, 姚淑娟, 路常宽, 高宝嘉. 绿盲蝽取食诱导赤霞珠冬芽全蛋白双向电泳体系的建立与优化[J]. 华北农学报, 2022, 37(1): 181-187.
[6]	吴红红, 段学粉, 郭仰东, 张喜春. *转录因子SlMYB-related 2对番茄耐冷性的影响*[J]. 华北农学报, 2022, 37(1): 35-41.
[7]	宋雅欣, 赵同科, 安志装, 马茂亭. 有机无机肥料配施对设施番茄产量及土壤养分含量的影响[J]. 华北农学报, 2021, 36(S1): 306-311.
[8]	王大刚, 陈圣男, 黄志平, 李杰坤, 吴倩, 胡国玉, 王维虎, 杨永庆. 大豆新品系抗SMV鉴定及分析[J]. 华北农学报, 2021, 36(S1): 326-332.
[9]	杨官凯, 武育芳, 曹行行, 王晓东, 张雪艳. 微咸水灌溉对玉米秸秆有机复合基质番茄生长及品质特性的影响[J]. 华北农学报, 2021, 36(S1): 81-88.
[10]	金凤媚, 薛俊, 孙海波, 郝志愚. 基于小RNA技术的天津地区番茄花叶病毒分子检测与基因组部分序列分析[J]. 华北农学报, 2021, 36(5): 176-183.
[11]	吴婷婷, 陈海龙, 黄俊, 梁毅, 张婷, 李博文, 赖怡帆, 邹玉莹, 车凡昊, 吴俊, 刘金灵, 刘雄伦. Pi9基因分子标记辅助选择改良水稻[J]. 华北农学报, 2021, 36(5): 191-197.
[12]	腾海艳, 徐丹. OsSP1基因编辑和过表达对水稻生长和干旱抗性的影响[J]. 华北农学报, 2021, 36(5): 1-9.
[13]	代惠洁, 程琳, 曹慧, 竺晓平, 赵静. 烟粉虱取食ToCV侵染番茄对其解毒酶和保护酶基因表达的影响[J]. 华北农学报, 2021, 36(4): 172-183.
[14]	钟婷婷, 郭诗芬, 卢文斌, 肖钢. 甘蓝型油菜抗根肿病KASP标记开发和利用[J]. 华北农学报, 2021, 36(4): 184-190.
[15]	程欣然, 蔡欣月, 岩温香, 牛江帅, 吴荣, 牛庭莉, 穆云静, 戴凌燕. *异源过表达Atvip1基因增强转基因高粱对盐碱胁迫的抗性*[J]. 华北农学报, 2021, 36(4): 1-9.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

N-3-羰基十四烷酰基高丝氨酸内酯诱导 番茄对灰霉病抗性机制初探

The Exploration of the Effect of N -3-oxo-tetradecanoyl Homoserine Lactone on the Resistance to Botrytis cinerea on Tomato

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

N-3-羰基十四烷酰基高丝氨酸内酯诱导番茄对灰霉病抗性机制初探