华北农学报 ›› 2016, Vol. 31 ›› Issue (S1): 135-139. doi: 10.7668/hbnxb.2016.S1.024

所属专题: 油料作物

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

大豆广谱抗源对SMV优势株系的抗性遗传和等位性分析

王大刚, 张磊, 吴倩, 胡晨, 胡国玉, 李杰坤, 黄志平   

  1. 安徽省农作物品质改良重点实验室, 安徽省农业科学院 作物研究所, 安徽 合肥 230031
  • 收稿日期:2016-10-15 出版日期:2016-12-28
  • 通讯作者: 黄志平(1969-),男,安徽枞阳人,研究员,硕士,主要从事大豆遗传育种研究。
  • 作者简介:王大刚(1979-),男,安徽霍邱人,副研究员,博士,主要从事大豆抗病遗传育种研究。
  • 基金资助:
    国家自然科学基金项目(31201235;31571687);安徽省自然科学基金项目(1308085QC59);安徽省农业科学院学科建设项目(15A0202)

Inheritance and Allelism Analysis of Resistance to Soybean mosaic virus Predominant Strains in Soybean

WANG Dagang, ZHANG Lei, WU Qian, HU Chen, HU Guoyu, LI Jiekun, HUANG Zhiping   

  1. Key Laboratory of Crop Quality Improvement of Anhui Province, Crop Institute of Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2016-10-15 Published:2016-12-28

摘要: 为了研究大豆广谱抗源对我国大豆花叶病毒优势株系SC3和SC7的遗传方式及抗源材料对SMV抗性基因间的等位性关系,利用广谱抗源科丰1号和齐黄1号与感病材料南农1138-2配制抗感及抗抗杂交组合,通过人工摩擦接种法进行鉴定。结果发现,接种株系SC3和SC7后,科丰1号和齐黄1号与南农1138-2配制抗感组合的F1均表现抗病,经卡方测验,F2抗感分离比例符合3:1,F2:3家系分离比例为1(抗):2(分离):1(感),说明这2个广谱抗源均有1对显性基因控制株系SC3和SC7的抗性;等位性测验结果表明2个抗抗组合的F1对SC3和SC7优势株系均表现抗病,F2分离比符合15(抗):1(感),说明科丰1号和齐黄1号对株系SC3和SC7的抗性基因不等位且独立遗传。进一步分析2个广谱抗源携带的抗性基因可以发现,科丰1号对株系SC3的抗性基因RSC3和齐黄1号对SC7株系的抗性基因RSC7Q可能位于大豆的2号和13号染色体上,为利用大豆广谱抗源进行抗SMV育种奠定了很好的基础。

关键词: 大豆, 大豆花叶病毒, 抗性遗传, 等位性

Abstract: In order to research the resistance the relationship among the resistance genes present in these two broad-spectrum resistance cultivars to Soybean mosaic virus (SMV) predominant strains SC3 and SC7 in China.Two broad-spectrum soybean resistant (R) cultivars Kefeng No.1 and Qihuang No.1 were crossed respectively with susceptible (S) cultivars Nannong 1138-2 and resistant parent was also crossed with each other. The experiments were performed by using the artificial friction way under greenhouse/net-house conditions.The F2 and F2:3 lines derived from the R×S exhibited a segregation pattern of 3R:1S and 1R:2segregating:1S ratio respectively when inoculated with the SMV strains SC3 and SC7.The results indicated that Kefeng No.1 and Qihuang No.1 carried single dominant resistant gene for both SC3 and SC7.The test of the allelism of the resistance genes to SC3 and SC7 showed that the F2 from R×R crosses exhibited a segregation pattern of 15R:1S.The results suggested that the resistance genes between Kefeng No.1 and Qihuang No.1 were not at the same locus.Additionally,we inferred that Kefeng No.1 carried the RSC3 gene maybe on chromosome 2 and Qihuang No.1 carried the RSC7Q gene on chromosome 13,these results are important for soybean resistance breeding programs.

Key words: Soybean, Soybean mosaic virus, Inheritance of resistance, Allelism

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引用本文

王大刚, 张磊, 吴倩, 胡晨, 胡国玉, 李杰坤, 黄志平. 大豆广谱抗源对SMV优势株系的抗性遗传和等位性分析[J]. 华北农学报, 2016, 31(S1): 135-139. doi: 10.7668/hbnxb.2016.S1.024.

WANG Dagang, ZHANG Lei, WU Qian, HU Chen, HU Guoyu, LI Jiekun, HUANG Zhiping. Inheritance and Allelism Analysis of Resistance to Soybean mosaic virus Predominant Strains in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 135-139. doi: 10.7668/hbnxb.2016.S1.024.

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