[<sup>60</sup>Co]γ射线诱导小麦Glu-1A等位基因突变体的品质差异与遗传规律

doi:10.7668/hbnxb.2016.S1.017

摘要/Abstract

摘要： 为了研究[⁶⁰Co]γ射线诱导小麦Glu-1A等位基因突变体的品质差异与遗传规律，用300 Gy的[⁶⁰Co]γ射线辐射皖麦50的干种子，用近红外品质测定和SDS-PAGE法，对获得的Glu-1A等位基因品质量差异进行了评价和遗传规律研究，结果表明：在M₂筛选到一个HMW-GS突变体，Glu-1ANull突变为Glu-1A1，突变率为0.15%。突变体与皖麦50杂交，在F₂品质性状有超亲现象，品质得分与吸水率和硬度之间相关显著。在F₁的正、反交，HMW-GS表现为双显性的Glu-1A1，侧交的Glu-1A1：Glu-1ANull符合1：1的分类规律，F₂的Glu-1A1：Glu-1ANull符合3：1的分类规律。突变体蛋白质含量增加，杂交后代有品质超亲现象，遗传表现符合孟德尔独立遗传和自由组合规律。

关键词: 小麦, [⁶⁰Co]γ射线, Glu-1A1, 品质, 遗传规律

Abstract: Inorder to study quality differences and inheritance of Glu-1A Alleles mutant of wheat induced by[⁶⁰Co]γ-ray,with 300 Gy of[⁶⁰Co]γ-ray radiation of dry wheat seeds of Wanmai 50,and with method of analysis of near infrared and SDS-PAGE,quality evaluation and differences was made and inheritance of Glu-1A Alleles mutant was studied.The results showed that one HMW-GS mutant was screened in M₂,Glu-1A subunit component changed from Glu-1ANull to Glu-1A1,and the mutation ratio was 0.15%.The quality traits of the mutant Wanmai 50 in F₂ had transgressive phenomena.There was significant correlation between quality score and water absorption,hardness and sedimentation.The HMW-GS of orthogonal and reciprocal cross of F₁ all showed double dominance of Glu-A1.The ratio of Glu-1A1 to Glu-1ANull was in line with 1:1 segregation rule in side-crosses,and was in line with 3:1 in F₂. In conclusion,the protein content of mutant increased,the hybrids had super-pro-quality,and the inheritance accorded with the Mendelian inheritance and free combination.

Key words: Triticum aestivum L., [⁶⁰Co]γ-ray, Glu-1A1, Quality, Inheritance

中图分类号:

S512.03

张从宇, 李文阳, 许峰, 闫素辉, 邵庆勤. [⁶⁰Co]γ射线诱导小麦Glu-1A等位基因突变体的品质差异与遗传规律[J]. 华北农学报, 2016, 31(S1): 97-100. doi: 10.7668/hbnxb.2016.S1.017.

ZHANG Congyu, LI Wenyang, XU Feng, YAN Suhui, SHAO Qingqin. Quality Differences and Inheritance of Glu-1A Alleles Mutant of Wheat Induced by[⁶⁰Co]γ-Ray[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 97-100. doi: 10.7668/hbnxb.2016.S1.017.

http://www.hbnxb.net/CN/Y2016/V31/IS1/97

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[⁶⁰Co]γ射线诱导小麦Glu-1A等位基因突变体的品质差异与遗传规律

Quality Differences and Inheritance of Glu-1A Alleles Mutant of Wheat Induced by[⁶⁰Co]γ-Ray

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[60Co]γ射线诱导小麦Glu-1A等位基因突变体的品质差异与遗传规律

Quality Differences and Inheritance of Glu-1A Alleles Mutant of Wheat Induced by[60Co]γ-Ray

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[⁶⁰Co]γ射线诱导小麦Glu-1A等位基因突变体的品质差异与遗传规律

Quality Differences and Inheritance of Glu-1A Alleles Mutant of Wheat Induced by[⁶⁰Co]γ-Ray