高表达转C<sub>4</sub>型<em>PEPC</em>基因水稻在低氮下诱导碳氮酶稳定光合作用

doi:10.7668/hbnxb.2015.04.017

华北农学报 ›› 2015, Vol. 30 ›› Issue (4): 95-100. doi: 10.7668/hbnxb.2015.04.017

所属专题： 水稻；生物技术；

高表达转C₄型PEPC基因水稻在低氮下诱导碳氮酶稳定光合作用

唐玉婷^1,2, 李霞², 陆巍¹, 魏晓东²

1. 南京农业大学生命科学学院, 江苏南京 210095;
2. 江苏省农业科学院粮食作物研究所, 江苏省优质水稻工程技术研究中心, 国家水稻改良中心南京分中心, 江苏南京 210014

收稿日期:2015-05-04 出版日期:2015-08-28
通讯作者: 李霞(1970-)女,广西桂平人,研究员,博士,主要从事水稻光合生理研究。陆巍(1971-)男,江苏无锡人,副教授,博士,主要从事植物光合作用研究。
作者简介:唐玉婷(1991-)女,安徽阜阳人,在读硕士,主要从事水稻光合生理研究。
基金资助:
国家自然科学基金项目(No.31371554);江苏省自主创新基金项目(CX[(14)5004]);江苏省自然科学基金项目(BK20130708)

Transgenic Rice with High Expression of C₄-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions

TANG Yu-ting^1,2, LI Xia², LU Wei¹, WEI Xiao-dong²

1. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center Rice Improvement, Nanjing 210014, China

Received:2015-05-04 Published:2015-08-28

摘要/Abstract

摘要： 为揭示高表达转玉米C₄-PEPC 基因水稻在低氮条件下的光合表现,采用高表达转玉米C₄-PEPC 基因水稻(PC) 与原种Kitaake(WT)为试验材料,通过盆栽试验,分别测定不同的氮素条件下(中氮300 kg/hm²,低氮65 kg/hm²), 开花后不同天数的SPAD值,光合参数以及碳氮关键酶活性。并在开花后50 d收获植株,记录产量因子。结果表明:低氮处理下的PC相对于WT来说,在开花后14,28 d,其净光合速率分别提高了13.10%(P <0.05)和29.29%(P <0.05)。同时,Rubisco羧化酶在开花后14,28 d时活性分别提高了67.86%和52.63%(P <0.05);硝酸还原酶在14,28 d时的活性分别提高79.49%(P <0.05)和17.96%;谷氨酰胺合成酶仅在开花后28 d时活性提高28.48%(P <0.05)。但是通过对产量进行分析,在低氮条件下,并未发现PC的产量与WT有明显差异。可见,PC通过诱导碳氮的关键酶活性的提高维持低氮条件下高净光合速率。

关键词: 高表达转C₄型 PEPC 基因水稻, 低氮, 光合特性

Abstract: The objective of this study was to reveal transgenic rice with high expression of maize C₄-PEPC photosynthetic performance under low nitrogen conditions,two rice genotypes including C₄-PEPC transgenic rice (PC)and untransgenic Kitaake rice(WT) in the pot experiments were chosen to measure their photosynthesis characteristics,SPAD values,PEPC,Rubisco,NR and GS activities of flag leaves at different flowering stage under different nitrogen applications(normal nitrogen 300 kg/ha,low nitrogen 65 kg/ha) and their yield component were investigated after harvest.The results showed that the net photosynthetic rate of PC compared with WT were increased by 13.10% (P <0.05) and 29.29% (P <0.05),respectively in the 14,28 d after flowering under low nitrogen treatment.At the same time,under low concentrations of nitrogen,relative to untransformed wild type rice,activity of Rubisco carboxylase of PC were increased by 67.86% and 52.63% (P <0.05) in the 14,28 d after flowering respectively,while activity of nitrate reductase of PC were increased 79.49% (P <0.05) and 17.96%.Meanwhile,activity of glutamine synthetase of PC was increased 28.48% (P <0.05) only in the 28 days after flowering comparing with WT.But we did not find significant differences in the yield of PC and WT under low nitrogen conditions.Therefore,PC maintain high net photosynthetic rate by inducing the activity of carbon and nitrogen key enzyme improvement under low nitrogen conditions.

Key words: High expression of maize C₄-PEPC transgenic rice, Low nitrogen, Photosynthesis

中图分类号:

唐玉婷, 李霞, 陆巍, 魏晓东. 高表达转C₄型PEPC基因水稻在低氮下诱导碳氮酶稳定光合作用[J]. 华北农学报, 2015, 30(4): 95-100. doi: 10.7668/hbnxb.2015.04.017.

TANG Yu-ting, LI Xia, LU Wei, WEI Xiao-dong. Transgenic Rice with High Expression of C₄-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 95-100. doi: 10.7668/hbnxb.2015.04.017.

http://www.hbnxb.net/CN/Y2015/V30/I4/95

参考文献

[1] Ku M B,Agarie S,Nomura M,et al.High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants[J].Nature Biotechnology,1999,l7(1):76-80.
[2] 焦德茂,李霞,黄雪清,等.转 PEPC 基因水稻的光合CO₂同化和叶绿素荧光特性[J].科学通报,2001,46(5):414-418.
[3] 焦德茂,匡廷云,李霞,等.转 PEPC 基因水稻具有初级CO₂浓缩机制的生理特点[J].中国科学(C辑),2003,33(1):33-39.
[4] Ren C G,Li X,Liu X L,et al.Hydrogen peroxide regulated photosynthesis in C₄-pepc transgenic rice[J].Plant Physiology and Biochemistry,2014,74:218-229.
[5] 魏晓东,李霞,郭士伟,等.氮素水平对转C₄光合基因水稻花期剑叶PSⅡ荧光特性的影响[J].华北农学报,2013,28(1):193-200.
[6] Li X,Wang C.Physiological and metabolic changes of transgenic rice plant with increased activity of phosphoenolpyruvate carboxylase during flowering stage[J].Acta Physiologiae Plantarum,2013,35(5):1503-1512.
[7] Li B,Li G,Kronzucker H J,et al.Ammonium stress in Arabidopsis:signaling,genetic loci,and physiological targets[J].Trends in Plant Science,2014,19(2):107-114.
[8] Chen P,Li X,Huo K,et al.Promotion of photosynthesis in transgenic rice over-expressing of maize C₄ phosphoenolpyruvate carboxylase gene by nitric oxide donors[J].Journal of Plant Physiology,2014,171(6):458-466.
[9] Li X,Cao K,Wang C,et al.Variation of photosynthetic tolerance of rice cultivars (Oryza sativa L.) to chilling temperature in the light[J].African Journal of Biotechnology,2010,9(9):1325-1337.
[10] Lilley R M,Walker D A.An improved spectrophotometric assay for ribulosebisphosphate carboxylase[J].Biochimica et Biophysica acta,1974,358(1):226-229.
[11] Eckardt N A,Portis Jr A R.Heat denaturation profiles of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase and the inability of Rubisco activase to restore activity of heat-denatured rubisco[J].Plant Physiology,1997,113(1):243-248.
[12] Hu Y,Schmidhalter U.Drought and salinity:a comparison of their effects on mineral nutrition of plants[J].Journal of Plant Nutrition and Soil Science,2005,168(4):541-549.
[13] Kraiser T,Gras D E,Gutiérrez A G,et al.A holistic view of nitrogen acquisition in plants[J].Journal of Experimental Botany,2011,62(4):1455-1466.
[14] 季本华,朱素琴,焦德茂.转玉米C₄光合酶基因水稻株系中的光合C₄微循环[J].作物学报,2004,30(6):536-543.
[15] Jiao D M,Huang X,Li X,et al.Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C₄ photosynthesis enzymes[J].Photosynthesis Research,2002,72(1):85-93.
[16] Parry M A J,Andralojc P J,Khan S,et al.Rubisco activity:effects of drought stress[J].Annals of Botany,2002,89(7):833-839.
[17] Bethke P C,Libourel I G L,Jones R L.Nitric oxide reduces seed dormancy in Arabidopsis[J].Journal of Experimental Botany,2006,57(3):517-526.
[18] Hu X,Neill S J,Tang Z,et al.Nitric oxide mediates gravitropic bending in soybean roots[J].Plant Physiology,2005,137(2):663-670.
[19] Martin A,Lee J,Kichey T,et al.Two cytosolic glutamine synthetase isoforms of maize are specifically involved in the control of grain production[J].The Plant Cell,2006,18(11):3252-3274.
[20] Obara M,Sato T,Sasaki S,et al.Identification and characterization of a QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice[J].Theoretical and Applied Genetics,2004,110(1):1-11.
[21] Tabuchi M,Abiko T,Yamaya T.Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.)[J].Journal of Experimental Botany,2007,58(9):2319-2327.
[22] 李学勇,钱前,李家洋.水稻分蘖的分子机理研究[J].中国科学院院刊,2003,18(4):274-276.
[23] Mcallister C H,Beatty P H,Good A G.Engineering nitrogen use efficient crop plants:the current status[J].Plant Biotechnology Journal,2012,10(9):1011-1025.
[24] 王鹏程,杜艳艳,宋纯鹏.植物细胞一氧化氮信号转导研究进展[J].植物学报,2009,44(5):517-525.
[25] Garcia-Mata C,Gay R,Sokolovski S,et al.Nitric oxide regulates K⁺ and Cl^- channels in guard cells through a subset of abscisic acid-evoked signaling pathways[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(19):11116-11121.
[26] Bouguyon E,Gojon A,Nacry P.Nitrate sensing and signaling in plants[J].Seminars in Cell & Developmental Biology,2012,23(6):648-654.
[27] Ho C H,Lin S H,Hu H C,et al.CHL1 functions as a nitrate sensor in plants[J].Cell,2009,138(6):1184-1194.
[28] Parker J L,Newstead S.Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1[J].Nature,2014,507(7490):68-72.
[29] Sun J,Bankston J R,Payandeh J,et al.Crystal structure of the plant dual-affinity nitrate transporter NRT1.1[J].Nature,2014,507(7490):73-77.
[30] Krouk G,Crawford N M,Coruzzi G M,et al.Nitrate signaling:adaptation to fluctuating environments[J].Current Opinion in Plant Biology,2010,13(3):266-273.
[31] Remans T,Nacry P,Pervent M,et al.The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(50):19206-19211.

选择文件类型/文献管理软件名称

选择包含的内容

高表达转C₄型PEPC基因水稻在低氮下诱导碳氮酶稳定光合作用

Transgenic Rice with High Expression of C₄-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘帅, 徐学欣, 赵金科, 曲文凯, 郝天佳, 孟繁港, 贾靖, 赵长星. 滴灌水肥一体化下施氮量与频次对夏玉米光合特性、保护酶活性及产量的影响[J]. 华北农学报, 2022, 37(5): 114-123.
[2]	包秀霞, 廉勇, 慕宗杰, 张欢. 干旱胁迫下不同来源多根葱根系及叶片光合特性的比较[J]. 华北农学报, 2022, 37(4): 122-127.
[3]	牛润芝, 朱长伟, 姜桂英, 杨锦, 罗澜, 申凤敏, 刘芳, 刘世亮. 豫北潮土区轮耕模式对小麦光合特性、产量及土壤养分的影响[J]. 华北农学报, 2022, 37(4): 182-189.
[4]	徐丽霞, 董雪, 张艾英, 郭二虎, 孙靓. 不同轮作模式对谷子生理生化指标和产量的影响[J]. 华北农学报, 2022, 37(3): 68-76.
[5]	周袁慧, 马尚宇, 王艳艳, 黄正来, 张文静, 樊永惠, 侯君佑, 盖盼盼, 单雅敬. 花后渍水对小麦旗叶光合和籽粒灌浆特性及产量的影响[J]. 华北农学报, 2021, 36(S1): 111-117.
[6]	李静怡, 王忍, 孟祥杰, 梁玉刚, 龚向胜, 黄璜, 魏海林. 免耕半固态直播对水稻剑叶生理特性的影响[J]. 华北农学报, 2021, 36(5): 87-98.
[7]	赵鑫. 氮素营养对苦荞光合特性及土壤酶活性的调控[J]. 华北农学报, 2020, 35(S1): 262-267.
[8]	高翠民, 杨永辉, 何方, 韩伟峰, 王小非, 武继承. 不同灌溉技术下水氮耦合对小麦光合特性、灌水利用特性及产量的影响[J]. 华北农学报, 2020, 35(5): 72-80.
[9]	周大虎, 瞿子扬, 聂丽云, 刘嘉龙, 傅军如, 唐文帮, 贺浩华. 优质地方品种鹅湖香稻耐低氮特性分析[J]. 华北农学报, 2020, 35(5): 132-139.
[10]	李欣欣, 石祖梁, 王久臣, 王飞, 徐志宇, 江荣风. 施氮量和种植密度对稻茬晚播小麦干物质积累及光合特性的影响[J]. 华北农学报, 2020, 35(5): 140-148.
[11]	翟锡姣, 刘婷婷, 曹世宇, 卫旭阳, 郑少文. CO₂加富及不同灌溉次数对菜豆生长及光合特性的影响[J]. 华北农学报, 2020, 35(2): 88-97.
[12]	刘斌祥, 程秋博, 周芳, 杜伦静, 李小龙, 孔凡磊, 袁继超. 种子大小与播种深度对玉米出苗、苗期光合特性与保护酶活性的影响[J]. 华北农学报, 2020, 35(2): 98-106.
[13]	王甜, 庞婷, 杜青, 陈平, 张晓娜, 周颖, 汪锦, 杨文钰, 雍太文. 田间配置对间作大豆光合特性、干物质积累及产量的影响[J]. 华北农学报, 2020, 35(2): 107-116.
[14]	路亚, 王春晓, 王丽丽, 于天一, 郑永美, 吴正锋, 李林, 王才斌. 花生幼苗对酸胁迫的生理响应及品种间差异[J]. 华北农学报, 2020, 35(1): 73-80.
[15]	孟宪敏, 季延海, 武占会, 储昭胜, 刘明池. 营养液浓度对封闭式槽培黄瓜品质、产量及光合特性的影响[J]. 华北农学报, 2019, 34(5): 153-162.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

高表达转C4型PEPC基因水稻在低氮下诱导碳氮酶稳定光合作用

Transgenic Rice with High Expression of C4-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

高表达转C₄型PEPC基因水稻在低氮下诱导碳氮酶稳定光合作用

Transgenic Rice with High Expression of C₄-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions