摘要： 为拓宽花生的耐盐基因资源,前期以平阳霉素为诱变剂进行了花生离体诱变,并在培养基中添加NaCl进行定向筛选,获得了一批NaCl耐性苗及其后代。对NaCl耐性植株M₃个体的荚果产量进行了测定,结果表明,有5个原始再生植株产生了3个以上大于60 g荚果重的M₃单株。用近红外仪对M₄种子的各品质性状进行测定,结果发现,其在蛋白质、油酸、亚油酸及脂肪含量发生了广泛变异,有7份变异材料的蛋白质含量超过30%,11份变异材料的脂肪含量超过55%。结合M₄种子在0.7%盐溶液中的发芽情况,筛选出几个综合了高产、高油、耐盐多个优良性状的个体。证明PYM离体诱变、NaCl定向筛选与近红外技术的有效结合,在花生中是一条新的有效的育种途径。

关键词: 花生, 平阳霉素, 定向筛选, 近红外技术, 品质

Abstract: To expand the salt-tolerant gene resources of peanut, we conducted in vitro mutagenesis with pingyangmycin(PYM)as the mutagen and directed screening on a medium containing NaCl in the previous experiment.In this study, pods of M₃ individual plants from those original NaCl-tolerant plants were collected and weighted after harvest and sun drying and the results indicated that 5 original HYP-tolerant, regenerated plants produced≥3 offspring with over 60 g/pods per plant.M₄ seeds were tested for quality by Near Infrared Spectroscopy, and the results indicated that there was substantial variation in the protein content, oleic acid, linoleic acid content, and oil content, including seven with higher protein content(>30%)and 11 individuals with higher oil content(>55%).According to the results of pod weight per plant and quality traits combined with a germination test with a 0.7% NaCl solution of M₄ seeds, we screened a few individuals with high yield, oil content and salt tolerance.We concluded that the use of PYM-based in vitro mutagenesis in combination with directed screening with NaCl and Near Infrared Spectroscopy technology is a new effective pathway for breeding of peanut.

Key words: Peanut, Pingyangmycin(PYM), Directed screening, Near infrared spectroscopy technology, Quality

中图分类号: 

• S565.01