丽江新团黑谷近等基因系孕穗期耐冷性生化性状的主成分分析及综合评价

doi:10.7668/hbnxb.2014.S1.050

华北农学报 ›› 2014, Vol. 29 ›› Issue (S1): 262-272. doi: 10.7668/hbnxb.2014.S1.050

所属专题： 生物技术；

丽江新团黑谷近等基因系孕穗期耐冷性生化性状的主成分分析及综合评价

杨树明^1,3, 曾亚文^1,2, 王荔⁴, 张素华¹, 杜娟¹, 杨涛¹, 普晓英¹, 杨晓梦¹

1. 云南省农业科学院生物技术与种质资源研究所, 云南昆明 650205;
2. 农业部西南农业基因资源与种质创制重点实验室, 云南昆明 650223;
3. 云南省农业生物技术重点实验室, 云南昆明 650223;
4. 云南农业大学农学与生物技术学院, 云南昆明 650201

收稿日期:2014-10-22 出版日期:2014-12-28
通讯作者: 曾亚文(1967-),男,云南嵩明人,研究员,博士,主要从事作物遗传育种及产业开发研究。
作者简介:杨树明(1973-),男,云南武定人,研究员,博士,主要从事作物种质资源、遗传育种及其生态环境研究。
基金资助:
国家自然科学基金项目(30160043;31060186);云南省自然科学基金项目(2010CD006);云南省技术创新人才培养项目(2011CI059;2012HB050);云南省农业生物技术重点实验室开放项目(2014001)

Principal Component Analysis and Comprehensive Evaluation on Biochemical Characteristics of Cold Tolerance at Booting Stage in the Near-isogenic Lines from Japonica Rice Lijiangxintuanheigu

YANG Shu-ming^1,3, ZENG Ya-wen^1,2, WANG Li⁴, ZHANG Su-hua¹, DU Juan¹, YANG Tao¹, PU Xiao-ying¹, YANG Xiao-meng¹

1. Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;
2. Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650223, China;
3. Agricultural Biotechnology Key Laboratory of Yunnan Province, Kunming 650223, China;
4. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China

Received:2014-10-22 Published:2014-12-28

摘要/Abstract

摘要： 为建立鉴定水稻孕穗期耐冷种质资源的生化指标,以云南孕穗期耐冷标准品种昆明小白谷、丽粳2号和半节芒及粳稻近等基因系(十和田⁴//丽江新团黑谷/十和田)的BC₄F₈、BC₄F₉群体105个家系及亲本为材料,在大田冷水胁迫及正常温度条件下,连续在2011年和2012年于水稻孕穗期分别测定剑叶、花药和籽粒的26个生化性状,采用主成分分析、隶属函数法、逐步回归分析和K-means聚类法进行指标筛选及耐冷性综合评价。结果表明,剑叶中叶绿素a、叶绿素b、总叶绿素、叶绿素c、POD活性、游离氨基酸,以及剑叶和花药中可溶性蛋白、可溶性糖、丙二醛、CAT活性、抗坏血酸、游离脯氨酸和籽粒中可溶性蛋白、可溶性糖、蛋白质和γ-氨基丁酸含量对耐冷性影响显著,可作为穗期耐冷性鉴定综合指标。采用隶属函数法获得低温胁迫下以剑叶、花药和籽粒中各生化性状为对象的综合耐冷D值,用K-means聚类法对各D值进行联合聚类,可将丽江新团黑谷NILs的105个株系划分为3类,其中强耐冷株系32株,中度耐冷10株,弱耐冷63株。

关键词: 水稻, 近等基因系, 孕穗期耐冷性, 生化鉴定指标, 主成分分析, 综合评价

Abstract: The biochemical evaluation system were established for screening and identifying species with cold tolerance,105 rice materials,including three standard varieties of strong cold resistance from Yunnan Province,the NILs BC₄F₈ and BC₄F₉ generation derived from parents japonica cultivars Lijiangxintuanheigu(donor parent) and Towada(recurrent parent),and their parents,were employed for evaluation of cold tolerance and screening of cold resistance index at booting stage.26 biochemical traits including leaves,anthers,and grains of tested materials were determined under cold water-stress and normal conditions in 2011,2012.Comprehensive assessment of cold resistance based on the principal component analysis,subordinate function values analysis,regression analysis and K-means clustering analysis.The results showed Chlorophyll a,Chlorophyll b,total Chlorophyll,Chlorophyll c content,peroxidase(POD) activity,free amino acid content of flag leaves;soluble protein,soluble sugar,malondiadehyde(MDA) content,catalase(CAT) activity,ascorbic acid(ASA),proline content(Pro) of flag leaves and anthers;soluble protein,soluble sugar,protein,γ-aminobutyric acid(GABA) content of grains significantly influenced the cold resistance.Thus could be used as comprehensive index for identification of cold resistance at the booting stage.The values cold resistance comprehensive evaluation(D) of biochemical traits including leaves,anthers,and grains of tested materials based on the subordinate function values was chosen as object under cold stress condition,through K-means clustering analysis,105 lines of population should be divided into 3 categories including stongly cold-tolerant lines 32,mediate cold tolerance lines 10,and cold-sensitive lines 63.

Key words: Rice, Near-isogenic lines, Cold tolerance at booting stage, Biochemical identification index, Principal component analysis, Comprehensive evaluation

中图分类号:

S515.03

杨树明, 曾亚文, 王荔, 张素华, 杜娟, 杨涛, 普晓英, 杨晓梦. 丽江新团黑谷近等基因系孕穗期耐冷性生化性状的主成分分析及综合评价[J]. 华北农学报, 2014, 29(S1): 262-272. doi: 10.7668/hbnxb.2014.S1.050.

YANG Shu-ming, ZENG Ya-wen, WANG Li, ZHANG Su-hua, DU Juan, YANG Tao, PU Xiao-ying, YANG Xiao-meng. Principal Component Analysis and Comprehensive Evaluation on Biochemical Characteristics of Cold Tolerance at Booting Stage in the Near-isogenic Lines from Japonica Rice Lijiangxintuanheigu[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 262-272. doi: 10.7668/hbnxb.2014.S1.050.

http://www.hbnxb.net/CN/Y2014/V29/IS1/262

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