摘要： 以油橄榄(品种为弗奥)一年生幼苗为试验材料,喷施66.7 mg/L的多效唑(PP₃₃₃)于油橄榄幼苗叶片表面,每株喷施量为100 mL,且设置对照(喷施相同量的蒸馏水),随后停止浇水,分别取干旱胁迫0,2,7 d的油橄榄幼苗叶片进行各项生理生化指标的测定及比较分析,以期探讨干旱胁迫下叶面喷施多效唑对油橄榄幼苗叶片细胞膜保护系统的影响。结果表明,当干旱胁迫(停止浇水7 d)使细胞膜受到显著破坏时,叶片喷施PP₃₃₃显著减轻了细胞膜损伤程度,其细胞膜稳定指数比对照(叶片喷施对应量的蒸馏水)高41.5%;研究结果还显示,干旱胁迫下叶面喷施PP₃₃₃相对减小了油橄榄叶片叶绿素含量、类胡萝卜素含量下降幅度,显著提高了脯氨酸和可溶性糖含量, 进一步提高了SOD、POD和CAT活性,抑制MDA含量、O₂^{- ·}产生速率和H₂O₂含量升高的幅度,提高了Vc和还原型谷胱甘肽这2种重要抗氧化物质的含量。可见,干旱胁迫下叶面喷施PP₃₃₃能通过相对提高干旱胁迫下叶片的抗氧化酶活性、抗氧化物质及渗透调节物质含量,减轻了光合色素含量的下降和活性氧、自由基的累积,由此加强了对油橄榄幼苗叶片细胞膜的保护。

关键词: PP₃₃₃, 油橄榄, 干旱胁迫, 细胞膜, 抗性

Abstract: Based on olive(variety Frantoio) annual seedlings as test materials,spraying 66.7 mg/L(PP₃₃₃) on the surface of olive seedling leaves,each spraying quantity to 100 mL,and setting a contrast respectively(spraying the same amount of distilled water),then stop water,drought stress were taken 0,2,and 7 days of olive leaves for the determination of the physiological and biochemical indexes and comparative analysis,in order to explore the influences of PP₃₃₃ on olive seedling leaves' cell membrane protective systems under drought stress were studied in this paper.The results showed that cell membrane was damaged significantly under drought stress(stop watering 7 d),but spraying PP₃₃₃ on the olive seedling leaves significantly reduced the damage degree of the cell membrane,the data showed that its cell membrane's stability index was 41.5% higher than the contrast(the same amount of distilled water sprayed on the leaves) after stop watering 7 d.The results also showed that spraying PP₃₃₃ on olive seedling leaves relatively reduced the reducing range of chlorophyll content and carotenoid content,increased the content of proline and soluble sugar significantly,further improved the activity of SOD,POD and CAT,inhibited the increasing range of MDA content,O₂^{- ·}producing rate and H₂O₂ content,and even improved the content of the two kinds of important antioxidants(Vc and reduced glutathione) under the drought stress significantly.So it can be concluded that through improving the activity of antioxidant enzymes,enhancing the content of antioxidants and osmotic regulation substances,reducing the decline of photosynthetic pigment content and the accumulation of active oxygen and free radicals,spraying PP₃₃₃ on the olive seedling leaves promoted the protection on the cell membranes of olive seedling leaves under the drought stress.

Key words: PP₃₃₃, Olea europaea, Drought stress, Cell membrane, Resistance

中图分类号: 

• S567.7