亚洲棉<i>GaPP2C24</i>基因的克隆及表达分析

doi:10.7668/hbnxb.20190833

华北农学报 ›› 2020, Vol. 35 ›› Issue (3): 33-39. doi: 10.7668/hbnxb.20190833

所属专题： 棉花；抗旱节水；生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

亚洲棉GaPP2C24基因的克隆及表达分析

蔡肖, 甄军波, 刘琳琳, 刘迪, 唐丽媛, 张素君, 李兴河, 王海涛, 刘存敬, 张香云, 张建宏, 迟吉娜

河北省农林科学院棉花研究所, 农业农村部黄淮海半干旱区棉花生物学与遗传育种重点实验室, 国家棉花改良中心河北分中心, 河北石家庄 050051

收稿日期:2019-12-30 出版日期:2020-06-28
通讯作者: 张建宏(1981-),男,河北唐海人,副研究员,硕士,主要从事棉花分子育种研究;迟吉娜(1977-),女,山东掖县人,研究员,博士,主要从事棉花功能基因组和资源创新研究。
作者简介:蔡肖(1982-),女,河北石家庄人,副研究员,博士,主要从事棉花抗逆分子生物学研究。
基金资助:
河北省农林科学院基本科研业务费项目（2018070301）；河北省科技计划项目（16226303D）；河北省农林科学院现代农业科技创新工程（2019-3-7）

Cloning and Expression Analysis of PP2C24 Gene from Gossypium arboreum L.

CAI Xiao, ZHEN Junbo, LIU Linlin, LIU Di, TANG Liyuan, ZHANG Sujun, LI Xinghe, WANG Haitao, LIU Cunjing, ZHANG Xiangyun, ZHANG Jianhong, CHI Jina

Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Key Laboratory of Cotton Biology and Genetic Breeding in Huanghuaihai Semiarid Area, Ministry of Agriculture, National Cotton Improvement Center Hebei Branch, Shijiazhuang 050051, China

Received:2019-12-30 Published:2020-06-28

摘要/Abstract

摘要： PP2C是一大类重要的蛋白磷酸酶，广泛参与不同的逆境胁迫响应。为了解PP2C基因在干旱响应中的功能，以亚洲棉石系亚1号为材料，利用RT-PCR方法从中克隆了GaPP2C24基因，并对该基因进行了生物信息学分析，荧光定量PCR研究了其在亚洲棉不同组织和干旱胁迫下的表达情况。结果表明，GaPP2C24基因CDS序列全长为1 251 bp，编码416个氨基酸。序列同源比对发现，GaPP2C24与其他植物的PP2C氨基酸序列有较高的相似性，其中与可可树（EOY33930.1）、黄麻（OMO91132.1）的相似性分别为85%，84%。进化分析表明，GaPP2C24与同属锦葵目的黄麻聚在一支，亲缘关系最近，与可可树的亲缘关系次之。实时荧光定量PCR结果表明，GaPP2C24基因在子叶、下胚轴、胚根、叶、茎和根均有表达，且在茎中的表达量最高，根中次之。GaPP2C24受干旱胁迫的诱导表达，其在根和叶片中的相对表达量在处理1 h后最高，分别达到对照的53.9，6.9倍，推测该基因在棉花干旱响应中有重要作用。

关键词: 亚洲棉, GaPP2C24, 基因克隆, 干旱胁迫, 表达分析

Abstract: PP2C is an important group of protein phosphatase, which is widely involved in different adverse stress responses. In order to understand the functions of cotton PP2C genes in drought response, GaPP2C24 gene was cloned from G. arboreum cultivar Shixiya-1 by RT-PCR. Bioinformatics analysis of this gene were carried out and its expression patterns in different tissues and in drought stress response were studied by quantitative Real-time PCR. The results showed that the CDS sequence of GaPP2C24 gene was 1 251 bp in length and encoded 416 amino acids. Multiple sequence alignment showed that GaPP2C24 had a high similarity with amino acid sequences of other plants. The similarity between protein GaPP2C24 and cacao tree (EOY33930.1) and jute (OMO91132.1) was 85% and 84%, respectively. Phylogenetic tree analysis revealed that GaPP2C24 was much closer to jute that of the same genus malva, followed by cacao tree. The results of Real-time quantitative PCR showed that the expression of GaPP2C24 could be detected in cotyledons, hypocotyls, radicles, leaves, stems and roots. Its expression level was the highest in stems, followed by roots. The expression of GaPP2C24 was induced by drought stress. The expression of GaPP2C24 in roots and leaves reached the highest level after 1 h of drought treatment, with 53.9 and 6.9 times of the control, respectively. The results suggested that GaPP2C24 gene played an important role in cotton drought response.

Key words: Gossypium arboreum, GaPP2C24, Gene clone, Drought stress, Expression analysis

中图分类号:

Q78
S562

蔡肖, 甄军波, 刘琳琳, 刘迪, 唐丽媛, 张素君, 李兴河, 王海涛, 刘存敬, 张香云, 张建宏, 迟吉娜. 亚洲棉GaPP2C24基因的克隆及表达分析[J]. 华北农学报, 2020, 35(3): 33-39. doi: 10.7668/hbnxb.20190833.

CAI Xiao, ZHEN Junbo, LIU Linlin, LIU Di, TANG Liyuan, ZHANG Sujun, LI Xinghe, WANG Haitao, LIU Cunjing, ZHANG Xiangyun, ZHANG Jianhong, CHI Jina. Cloning and Expression Analysis of PP2C24 Gene from Gossypium arboreum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 33-39. doi: 10.7668/hbnxb.20190833.

http://www.hbnxb.net/CN/Y2020/V35/I3/33

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亚洲棉GaPP2C24基因的克隆及表达分析

Cloning and Expression Analysis of PP2C24 Gene from Gossypium arboreum L.

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亚洲棉GaPP2C24基因的克隆及表达分析

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