摘要： 为了探讨苹果自交亲和的发生机理，根据保守氨基酸序列“FTQQYQ”和“anti-1/WⅠPNV”设计了苹果花柱S基因的通用引物：P1：5’-TTTACGCAGCAATATCAG-3’；P2:5’-ACGTTCGGCCAAATA/CATT-3’，根据GeneBank登陆的花粉SLF1(DQ422810)、SLF9(AB270792)基因设计了花粉S基因的引物分别为：P SLF1(上游5’-3’：CTGGTGGTGTTCTTTCCTGTGTAT；下游5’-3’：ACTTAACTCTTCCCTCAACTCA)和P SLF9(上游5’-3’CAGGTGCGTGAAAGTGAAA；下游5’-3’：TGAGCCAAGCATAA-GAACCT)，以自交亲和的苹果品种早红香为试验材料，利用PCR-RFLP、克隆、测序等方法研究早红香苹果发生自交亲和的分子性状。结果表明：自交亲和的早红香苹果2条花柱S基因没有发生变异。经BLAST比较，由花粉S基因引物PSLF1和PSLF9扩增得到的花粉S基因的DNA序列，与MdSLF2和MdSLF9基因DNA序列的相似性分别为94%和96%，推导氨基酸序列相似性分别为94５和97%。由P SLF1扩增得到的花粉S基因SLF1？与MdSLF1的DNA序列相似性仅为83%，且二者不一致的碱基分布于整个DNA序列中，自交亲和苹果品种早红香花粉SLF1？基因可能变异为了MdSLF2。

关键词: 自交不亲和, 苹果, 花柱S基因, 花粉S基因

Abstract: In order to discuss the mechanism of apple self-compatibility,the primer P1:5-'TTTACGCAGC AATATCAG-3'and P2:5-'ACGTTCGGCCAAATA/CATT-3'of apple style S gene were designed according to the conservative amino acid sequence,the primer P SLF1(upriver 5-'3':CTGGTGGTGTTCTTTCCTGTGTAT;backward position 5-'3':ACTTAACTCTTCCCTCAACTCA) and P SLF9 (upriver 5-'3 'CAGGTGCGTGAAAGTGAAA;backward position 5-'3 ':TGAGCCAAGCATAAGAACCT) of apple pollen S gene were designed according to the SLF1 (DQ422810) and SLF9 (AB270792) in GeneBank,the molecular character of self-compatible apple Zaohongxiang was studied in the method of PCR-RFLP,clone and sequence.The result showed that:tow style S gene of self-compatible apple Zaohongxiang did not variation.DNA sequence of pollen S gene which was amplified through pollen S gene primer P SLF1 and P SLF9 compared with the sequence of MdSLF2 and MdSLF9 had a high similarity,which arrived at 94% and 96%,amino acid similarity was 94% and 97%.The similarity of Pollen S gene SLF1? which was amplified through P SLF1 and DNA sequence of MdSLF1 was 83%,and the differ bases were distributed in the whole DNA sequence,so that pollen S gene SLF? could changed into MdSLF2.

Key words: Self-compatible, Apple, Style S gene, Pollen S gene

中图分类号: 

• Q78

• S66.1