ATP是构建类似C4水稻的重要限制因素

doi:10.7668/hbnxb.2009.04.004

华北农学报 ›› 2009, Vol. 24 ›› Issue (4): 17-22. doi: 10.7668/hbnxb.2009.04.004

所属专题： 水稻；

ATP是构建类似C4水稻的重要限制因素

张边江¹, 凌丽俐², 陈全战¹, 华春¹, 焦德茂³

1. 南京晓庄学院, 生命科学系, 江苏南京 211171;
2. 西南大学, 柑橘研究所, 重庆, 400712;
3. 江苏省农业科学院, 江苏南京 210014

收稿日期:2009-05-08 出版日期:2009-08-28
通讯作者: 焦德茂(1937-),男,研究员,主要从事作物生理学研究。
作者简介:张边江(1979-),男,江苏东海人,讲师,博士,主要从事作物生理研究.
基金资助:
国家转基因专项(16100004);江苏省教育厅高校自然基金项目(08KJD180012);南京晓庄学院生态学重点学科(2005NXY01)

A Key Limited Factor ATP of Constructing C4-like Rice

ZHANG Bian-jiang¹, LING Li-li², CHEN Quan-zhan¹, HUA Chun¹, JIAO De-mao³

1. Life Department of Nanjing Xiaozhuang University, Nanjing 211171, China;
2. Citrus Research Institute, Southwest University, Chongqing 400712, China;
3. Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2009-05-08 Published:2009-08-28

摘要/Abstract

摘要： 为了分析ATP是否是转PEPC+PPDK基因水稻或转 PEPC+PPDK+ME基因水稻CO2浓缩过程的限制因素,以原种和不同转C4光合酶基因水稻为材料,测定了C4光合酶活性、光合速率以及活性氧代谢有关指标,结果表明:原种中具有全套的C4光合酶,但活性很低,而不同转C4光合酶基因水稻高表达了相应的C4酶活性.在高光条件下,与原种相比较,转PPDK基因水稻的光合速率未增加;转ME基因水稻的光合速率降低了.1%;转PEPC+PPDK双基因水稻与转PEPC基因水稻相近.ATP和ATP激活剂NaHSO3处理后,可显著提高转PEPC+PPDK双基因水稻和转PEPC+PPDK+ME基因水稻的光合放氧速率,达到玉米的80.7%,显现出类似C4的光合特点,表明ATP是构建类似C4水稻的重要限制因素.光氧化条件下,转PEPC+PPDK+ME基因水稻的耐光氧化能力得到进一步的增强.这些结果为构建C4水稻提供了技术途径.

关键词: 转基因水稻, 光合特性, C4光合循环, 三磷酸腺苷(ATP)

Abstract: With untransformed rice and transgenic rices with different C4 photosynthesis genes as the materials, the activity of C4 photosynthesis enzymes, the net photosynthetic rate ( Pn) and the indexes of act ive oxygen metabolism were determined.The activities of C4 photosynthesis enzymes in untransformed rice were very low, while those of corresponding enzymes in different transgenic rices were all highly expressed. The Pn of the PPDK transgenic rice was not increased, that of the ME transgenic rice was decreased by 6. 1%, that of the transgenic ricewith PEPC and PPDK genes( CK) was simi?? lar with that of the PEPC transgenic rice under high light intensity. After treatment with ATP and its activator NaHSO3, the photosynthetic rate of O2 evolution were significant ly increased in the CK transgenic rice and the transgenic rice with PEPC, PPDK andME genes( CKM), which reached 80. 7% of that in maize and exhibited photosynthetic character of C4?? like plants.This indicated that ATP is the key limited factor for construct ing C4??like rice. In addition, after MV( artificial photooxidative activator) treatment, the CKM transgenic ricewas more tolerant to photooxidative stress.These results would provide a new technical approach to constructing C4??like rice.

Key words: Transgenic rice, Photosynthetic characteristics, C4 photosynthetic cycle, Adenosine triphosphate(ATP)

中图分类号:

张边江, 凌丽俐, 陈全战, 华春, 焦德茂. ATP是构建类似C4水稻的重要限制因素[J]. 华北农学报, 2009, 24(4): 17-22. doi: 10.7668/hbnxb.2009.04.004.

ZHANG Bian-jiang, LING Li-li, CHEN Quan-zhan, HUA Chun, JIAO De-mao. A Key Limited Factor ATP of Constructing C4-like Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 17-22. doi: 10.7668/hbnxb.2009.04.004.

http://www.hbnxb.net/CN/Y2009/V24/I4/17

参考文献

[1] Ku M S B,Sakae A,Mika N,et al.High level expression of maize phosphoenopyruvate carboxylase in transgenic rice plants[J].Nat Biotechnol9997:76-80.
[2] Fukayama H,Tsuchida H,Agarie S,et al.Significant accumulation of C4-specific pyruvate,orthophosphate dikinase in a C3 plant rice[J].Plant Physio,200127:1136-1146.
[3] Tsuchida H,Tamai T,Fukayama H,et al.High level expression of C4-specific NADP-malic enzyme in leaves and impairment of photoautotrophic growth in a C3 plant,rice[J].Plant Cell Physiol,2001,42:138-145.
[4] Jiao D M,Li X,Huang X Q,et al.The characteristics of CO2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice[J].Chin Sci Bull,2001,46:414-418.
[5] Chi W,Jiao D M,Li X,et al.Photosynthetic characteristics of transgenic rice plants overexpressing maize phosphoenopyruvate carboxylase[J].Acta Bot Sin,2001,43:657-660.
[6] 黄雪清,焦德茂.转C4光合酶基因水稻株系的抗光氧化特性[J].植物生理学报,2001,27(5):393-400.
[7] Jiao D M,Li X,Ji B H.Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition[J].Photosynthetica,2005,43:501-508.
[8] Jiao D M,Kuang T Y,Li X,et al.Physiological characteristics of theprimitive CO2 concentrating mechanism in PEPC transgenicrice[J].Science in China,2003,46:438-446.
[9] Ji B H,Zhu S Q,Jiao D M.A limited photosynthetic C4-microcycle and its physiological function in transgenic rice plant expressing the Maize PEPC gene[J].Acta Bot Sin,2004,46(5):542-551.
[10] Jiao D M,Hang X Q,Li X,et al.Photosynthetic characteristics and tolerance to photooxidation of transgenic rice expressing C4 photosynthesis enzymes[J].Photosynth Res,2002,72:85-93.
[11] Ji B H,Tan H H,Zhou R,et al.Promotive effect of low concentrations of NaHSO3 on photophosphorylation and photosynthesis in phosphoenolopyruvate carboxylase transgenic rice leaves[J].Acta Bot Sin,2005,47(2):178-186.
[12] Chi W,Zhou J S,Zhang F,et al.Photosynthetic features of transgenic rice expressing sorghum C4 type NADP-ME[J].Acta Bot Sin,2004,46(7):873-882.
[13] Gonzalez D H,Iglesias A A,Andeo C S.On the regulation of phosphoenolpyruvate carboxylase activity from maize leaves by L-malate:effect of pH[J].Plant Physiol98416:425-429.
[14] Hatch M D,Sack C R.Pyruvate,Pi dikinase from leaves[J].Meth Enzymol975,42:212-219.
[15] 陈景治,陈冬兰,吴敏贤.高粱和小麦叶片苹果酸酶某些特性比较[J].植物生理学报981,7(4):345-350.
[16] 李斌,陈冬兰,施教耐.高粱NADP苹果酸脱氢酶的纯化及其分子特性[J].植物生理学报9943(2):113-121.
[17] 陈甲瑞,梁银丽,周茂娟,等.免耕和施肥对玉米光合速率的影响[J].水土保持研究,20063(6):72-74.
[18] Giannopolitis C N,Ries S K.Superoxide dismutase:I.Occurrence in higher plants[J].Plant Physiol977,59:309-314.
[19] Kochba J,Lave E S,Spiegel-Roy P.Difference in peroxidase activity and isoenzymes in embryogenic and non-embryogenic shamouti orange ovular callus lines[J].Plant Cell Physiol9778:463-467.
[20] 王爱国,罗广华.植物超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯990,6:55-57.
[21] Heath R L,Packer L.Photoperoxidation in isolated chloroplast.1.Kinetics and stoichiometry of fatty acid peroidation[J].Arch Biochem Biophys96825:189-198.
[22] Dennis N.Agricultural research:Consortium aims to supercharge rice photosynthesis[J].Science,2006,28(313):423.
[23] Freitag H,Stichler W.A remarkable new leaf type with unusual photosynthetic tissue in a central Asiatic genus of Chenopodiaceae[J].Plant Biol,2000,2:154-160.
[24] Huang X Q,Jiao D M,Chi W,et al.Characteristics of CO2 exchange and chlorophyll fluorescence of transgenic rice with C4 genes[J].Acta Bot Sin,2002,44(4):405-412.
[25] 崔继林.光合作用与生产力[M].南京:江苏科学技术出版社,2000:347.
[26] Mikio N,Fang Q,Lisa A B,et al.Laser-capture microdissection,a tool for the global analysis of gene expression in specific plant cell types:identification of genes expressed differentially in epidermal cells or vascular tissues of maize[J].The Plant Cell,20035:583-596.

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