华北农学报 ›› 2009, Vol. 24 ›› Issue (4): 23-25. doi: 10.7668/hbnxb.2009.04.005

所属专题: 小麦

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长片段小麦细菌人工染色体DNA亚克隆文库的构建

高双成, 王世华, 施江, 孔祥生, 史国安   

  1. 河南科技大学, 农学院, 洛阳市牡丹生物学重点实验室, 河南洛阳 471003
  • 收稿日期:2009-02-23 出版日期:2009-08-28
  • 作者简介:高双成(1976-),男,山西夏县人,讲师,主要从事植物分子生物学研究.
  • 基金资助:
    河南科技大学人才科学研究基金(09001163);河南科技大学科学研究基金(2008zy038)

Construction of Subclone Library for Wheat Megabase BAC DNA

GAO Shuang-cheng, WANG Shi-hua, SHI Jiang, KONG Xiang-sheng, SHI Guo-an   

  1. Agricultural College, Henan University of Science and Technology, Luoyang Key Laboratory of Tree Peony Biology, Luoyang 471003, China
  • Received:2009-02-23 Published:2009-08-28

摘要: 为了加快小麦基因组的测序,以Langdon库中的一个BAC克隆(BAC790O10)为材料,利用HydroShear DNA剪切仪将其打断,产生许多大小不同的DNA随机片段,回收其中~5 kb的片段,并将这些片段随机克隆入TOPO载体,构建了适合于鸟枪法测序的亚克隆文库.

关键词: 小麦, BAC克隆, 亚克隆文库

Abstract: BAC subclone library is an important platform for sequencing the whole genome or some specific regions. In order to accelerate the process of sequencing the wheat genome, a BAC clone (BAC790010) fromLangdon librarywas selected as material, and then was broken by DNA shearing instrument to produce many DNA fragments with different sizes, fromwhich the 3- 5 kb DNA fragments were retrieved and cloned to TOPO vector and then the subclone librarywas constructed that was suitable for shotgun sequencing. This BAC subclone librarywould provide a platformfor gene cloning and genome research of wheat.

Key words: Wheat, BAC clone, Subclone library

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引用本文

高双成, 王世华, 施江, 孔祥生, 史国安. 长片段小麦细菌人工染色体DNA亚克隆文库的构建[J]. 华北农学报, 2009, 24(4): 23-25. doi: 10.7668/hbnxb.2009.04.005.

GAO Shuang-cheng, WANG Shi-hua, SHI Jiang, KONG Xiang-sheng, SHI Guo-an. Construction of Subclone Library for Wheat Megabase BAC DNA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 23-25. doi: 10.7668/hbnxb.2009.04.005.

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