摘要： 以魔芋颗粒状愈伤组织为试材,进行了液体培养及植株再生诱导的探讨。结果表明,MS+BA 1.0 mg/L+2,4-D 0.2 mg/L+蔗糖30 g/L+琼脂6.0 g/L培养基适合从芽鞘诱导颗粒状愈伤组织。获得的愈伤组织切割成0.3cm左右在MS+BA 0.05 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+PVP 1.0 g/L液体培养基中振荡培养,建立液体培养体系。液体培养材料的生长曲线呈上升的半抛物线型,从第6天到第12天期间增重占整个培养周期增重的80%以上,随着液体培养材料的生长,培养液pH值上升。将液体培养12 d的材料转到1/2MS+BA 0.6 mg/L+NAA 0.1 mg/L+葡萄糖30 g/L+维生素C100 mg/L+琼脂6.0 g/L培养基上,进行愈伤组织的分化培养;然后将长到出叶期的大芽切割转入到1/2MS+NAA 0.1 mg/L+蔗糖30 g/L+琼脂6.0 g/L生根培养基诱导生根,形成再生植株。

关键词: 魔芋, 液体培养, 植株再生

Abstract: :The granular calli of konjac was employed for study of liquid culture and plant regeneration.The results showed that MS +BA 1.0 mg/L +2, 4-D 0.2 mg/L + sugar 30g/L + agar 6.0 g/L couldefficiently induce granularcalli from the bud.Obtained calli were cut about 0.3 cm and then subsequently cultured in MS +BA 0.05mg/L+NAA 0.1 mg/L+sugar 30 g/L +PVP(PolyVinyl-Pyrrolidone)1.0 g/L for establishing liquid culture system.The growth of liquid culture material showed a parabola curve, the weight gain for more than 80% of entire culture period from the sixth day to the twelfth day.In addition, pH value of medium increased during the culture process.The material that was cultured twelve days turned to 1/2MS +BA 0.6 mg/L +NAA 0.1 mg/L +glucose 30 g/L +vitamin C100 mg/L +agar 6.0 g/L for differentiation culture.Big buds that the leaf expects were cut and put on 1/2MS + +NAA 0.1 mg/L + sugar 30 g/L+agar 6.0 g/L for inducing root, eventually the regenerative plantlets were formed.

Key words: Konjac, Liquid culture, Plant regeneration

中图分类号: 

• S632.9