摘要： 以基因枪轰击后的6个大麦品种的幼胚为材料,研究了不同质量浓度的ABA,2,-D,ZT和IAA对胚幼的出愈率、愈伤组织分化特性和植株再生能力的影响.结果表明:用1.0 mg/L的ABA在诱导培养基上处理幼胚能大大降低胚芽鞘发生率,并对出愈率和胚性愈伤组织的形成无不良影响;经 1.0mg/L的2,-D诱导产生的胚性愈伤组织转到分化培养基上以后,芽分化率可达8%～17%,而.0 mg/L 2,-D处理芽分化率极低.1.0 mg/L ZT与0.1 mg/L IAA配比能降低分化过程中不定根的发生率,有利于芽的分化.通过植株再生系统的优化,6个大麦品种的转化幼胚植株再生率达到2%～8%.
关键词:
基因枪,
大麦幼胚,
植物激素,
愈伤组织,
植株再生
Abstract: Effects of different ABA,2,4-D concentrations and ZT and IAA ratio on callus induction and plant regeneration of barley immature embryos,bombed by microprojectile,were studied.The results demonstrated that 1.0 mg/L ABA could effectively inhibit coleoptile growth of immature embryo without negative effects.Frequency of bud differentiation was up to 8%～17% from embryonic callus induced by the treatment of 1.0 mg/L 2,4-D,while few buds induced by 4.0 mg/L 2,4-D.Desirable combination of ZT and IAA for bud differentiation was 1.0 mg/L ZT and 0.1 mg/L IAA.According to optimum hormone concentrations,plant regeneration frequency of bombed immature embryos from six barley varieties were at a range of 2%-8%.
Key words:
Microprojectile,
Barley immature embryos,
Hormone,
Callus,
Plant regeneration
中图分类号:
刘雷, 尹钧, 任江萍, 李会勇, 李磊. 基因枪轰击后大麦幼胚的组织培养及植株再生研究[J]. 华北农学报, 2003, 18(4): 1-4. doi: 10.3321/j.issn:1000-7091.2003.04.001.
LIU Lei, YIN Jun, REN Jiang-ping, LI Hui-yong, LI Lei. Tissue Culture and Plant Regeneration of Barley Immature Embryos Bombed by Microprojectile[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(4): 1-4. doi: 10.3321/j.issn:1000-7091.2003.04.001.