摘要： 用野生马齿苋的种子得到无菌苗。以无菌苗的下胚轴和子叶为外植体，在18个不同浓度6-BA，2，4-D，NAA和KT组合的MS培养基上进行愈伤组织诱导、不定芽分化和增殖及生根的培养。得到诱导马齿苋愈伤组织的最佳条件是：初代培养基：MS+3%蔗糖+6-BA 1 mg/L+2，4-D 1 mg/L；丛生芽增殖培养基：MS+3%蔗糖+KT 3 mg/L+NAA 0.5 mg/L；生根培养基：MS+3%蔗糖。

关键词: 马齿苋, 组织培养, 植株再生

Abstract: Asepsis plantlets were obtained from seeds of wildness Portulaca oleracea L.. Cotyledon and hypocotyle-donary axis were used as explants to induce calli and differentiate shoots and roots in the MS medium containing 18 different concentrations of 6-BA, 2, 4-D, NAA, and KT. From data obtained the best inducement condition of Portulaca oleracea L. calli tissue: prime medium: MS+3% sucrose+6-BA 1 mg/L+2, 4-D 1 mg/L; shoots proliferation medium: MS+3% sucrose+KT 3 mg/L+NAA 0.5 mg/L; crude root substrate: MS+3% sucrose.

Key words: Portulaca oleracea L., Tissue Culture, Plant Regeneration

中图分类号: 

• S68