摘要： 以茶花凤仙风干种子为材料,研究磷离子注入后对其组织培养的生物学效应.研究表明,注入磷离子各处理的种子萌发率和试管苗株高均高于对照组,且随处理剂量的提高萌发率降低,平均株高增加;处理组试管苗分化率和生根率均低于对照组;低能磷离子注入能够诱导凤仙组培苗试管内开花,处理剂量为1×1016 ions/cm2时成花率最高.
关键词:
离子注入,
茶花凤仙,
组织培养,
试管成花
Abstract: The study was carried out by using dry seeds as a treatment material and phosphor ion as an inducing source on Impatiens balsamena offspring. The results showed that the germinant rate,plantlet height of phosphor ion treat- ment were higher than control in vitro, and with dosage rising the germinant rate reduced,while the plantlet height in- creased; the differentiation rate and rooting rate of the treated material were lower than control in vitro; the P+ion treat- ment could induce Impatiens balsamena flowering in vitro,and the impregnation with 1× 1016ions/cm2was most benefi- cial to flower.
Key words:
Ion implantation,
Impatiens balsamina,
Tissue culture,
Flower formation in vitro
中图分类号:
孙宁, 张磊, 赵新海, 刘玉芹, 陈小强, 孟庆良, 孙永健. 离子注入对凤仙试管苗组织培养和成花的影响[J]. 华北农学报, 2009, 24(3): 159-161. doi: 10.7668/hbnxb.2009.03.034.
SUN Ning, ZHANG Lei, ZHAo Xin hai, LIU Yu qin, CHEN Xiao qiang, MENG Qing liang, SUN Yong jian. Effects of P+ ion Implantation on Culture and Flower Formation of Impatiens balsamina in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 159-161. doi: 10.7668/hbnxb.2009.03.034.