摘要： 以芹菜为研究材料,研究了萌发,生长,分化,生根,培养条件等因素对组培苗的生长影响。结果表明:收获1年的芹菜种子,用10mg/LGA3浸泡12h后,以0.1%HgCl2消毒90s,再铺种于含10mg/LGA3的MS中,6d后萌发,在20℃下14d后萌发率可达到70%以上,28℃时萌发率明显降低;以MS为基本培养基;1.0mg/LBA+1.0mg/LTDZ使其分化率达到100%,30d后平分化数达到5个;单独使用TDZ时,1.0mg/LTDZ的综合效果最好;0.01mg/LNAA下根的诱导率是100%,14d后平均生根数达到7.2。培养条件以20℃为宜。
关键词:
芹菜,
休眠,
GA3,
TDZ(Thidiazuron),
组织培养
Abstract: This study discussed the effect of Gibberellin( GA3) on dormant celery seeds, and TDZ on proliferation ofcelery.The result showed that celery dipped in 10 mg/L GA3 and plated onMS with 10mg/ L GA3 performed better( morethan 70% in 20℃) than these with no disposal on the germinat ion.Temperature is another factor during germination, andin high temperature( > 25℃) , the germination rate were restrained. Choose MS medium as the basic medium, with 1. 0mg/ L BA+ 1. 0 mg/ L TDZ, the differentiation rate reached 100% , and the shoot number achieved 5. With 0. 01 mg/ LNAA, the induce rate of roots achieved 100%, and the average roots per seedling reached 7. 2.
Key words:
Celery,
Dormancy,
Gibberellin(GA3),
TDZ( thidiazuron),
Tissue Culture
中图分类号:
YOUYa-wei，WANGYu-zhen, LIXia. The Effect of Gibberellin(GA3) on the Dormancy of Celery( Apium graveolens L. ) and the Effect of TDZ on the Proliferation of Celery[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.015.