小麦胚休眠的蛋白质组分析

doi:10.7668/hbnxb.2009.02.001

摘要/Abstract

摘要： 为探讨小麦种子休眠解除的机制,利用蛋白质组学方法比较解除休眠前后的小麦胚在ABA和H₂O₂中的蛋白质表达情况.H₂O₂能打破种子休眠,而ABA则使种子保持休眠状态.比较2D图谱后,利用MALD1-TOF-MS鉴定了10个在不同处理间表达丰度差异显著的蛋白点,其中一些蛋白在已知的种子萌发中起重要作用.种子休眠的解除是一个复杂的发育过程,涉及环境胁迫反应(胚发育后期丰度蛋白、NAD(P)H脱氢酶)、细胞循环(生长素反应蛋白)、信号转导(钙调素相关蛋白)和贮藏蛋白(2S种子清蛋白)代谢等生理活动.为研究H₂O₂打破小麦休眠的分子机理提供了重要参考.

关键词: 小麦, 休眠, ABA, 过氧化氢, 蛋白质组

Abstract: A proteomic approach was used to analyze the mechanisms of dormancy breaking in wheat embryos and the participation of hydrogen peroxide (H₂O₂) and abscisic acids (ABA) in this process. The dormant and non-dormant embryos were dissected and treated with water,1 % H₂O₂ and 25μmol/ L ABA solution. ABA delayed,whereas H₂O₂ promoted seed dormancy breaking. 2D maps for water,ABA and H₂O₂ were established. Ten spots,showing significant changes in expression level,were identified by MALDI2TOF2MS. Some of them had previously been shown to play a role during dormancy breaking or germination in several plant species.We conclude that the mechanism of seed dormancy breaking is acomplex developmental processes,involves in environment stress (such as LEA,NAD(P) H dehydrogenase),cell cycle activity(auxin-responsive protein IAA19),signal transduction (calmodulin2related protein) and seed storage protein (2S albu-min-like protein),etc.Altogether,the results provided important information on the molecular mechanisms of breaking dormancy in the wheat seeds by H₂O₂ application.

Key words: Wheat, Dormancy, ABA, Hydrogen peroxide, Proteome

中图分类号:

S512.03

孙果忠, 张秀英, 闫长生, 肖世和. 小麦胚休眠的蛋白质组分析[J]. 华北农学报, 2009, 24(2): 1-7. doi: 10.7668/hbnxb.2009.02.001.

SUN Guo-zhong, ZHANG Xiu-ying, YAN Chang-sheng, XIAO Shi-he. Proteomic Analysis of Embryonic Dormancy in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 1-7. doi: 10.7668/hbnxb.2009.02.001.

http://www.hbnxb.net/CN/Y2009/V24/I2/1

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