低温锻炼诱导葡萄幼苗的耐热性过程中sHSP17.6在叶片中的亚细胞定位

doi:10.7668/hbnxb.2009.02.002

华北农学报 ›› 2009, Vol. 24 ›› Issue (2): 8-11. doi: 10.7668/hbnxb.2009.02.002

所属专题： 葡萄；

低温锻炼诱导葡萄幼苗的耐热性过程中sHSP17.6在叶片中的亚细胞定位

张俊环¹, 黄卫东²

1. 中国农业大学, 食品科学与营养工程学院, 北京 100083;
2. 北京市农林科学院,林业果树研究所,北京 100093

收稿日期:2008-12-09 出版日期:2009-04-28
通讯作者: 黄卫东( 1961- ), 男, 广东龙门人, 教授, 博士生导师, 主要从事果树生理与分子生物学研究。
作者简介:张俊环( 1974- ), 女, 山东菏洋人, 博士, 主要从事果树逆境的细胞与分子生物学机制研究.
基金资助:
国家自然科学基金资助项目(30471192)

Cold Acclimation Induce the Changes in the Subcellular Distribution of Small Heat Shock Protein 17.6 in Young Grape Leaves During Heat Stress

ZHANG Jun-huan¹, HUANG Wei-dong²

1. College of Food Science and Nutritional Engineering,China Agricultural University, Beijing 100083, China;
2. Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China

Received:2008-12-09 Published:2009-04-28

摘要/Abstract

摘要： 为了探讨热激蛋白在低温锻炼诱导的耐热性形成过程中的保护功能,进一步分析葡萄幼苗对温度逆境产生交叉适应性的细胞学机制,试验以葡萄(Vitis vinifera L.cv.Jingxiu)幼苗为试材,采用胶体金免疫电镜定位技术,定位观察了小分子热激蛋白17.6(sHSP17.6)在葡萄叶片中的亚细胞分布情况,结果表明,低温锻炼预处理可增强其在高温胁迫条件下的表达水平,细胞核和叶绿体中代表sHSP17.6的免疫金颗粒密度比相应的对照细胞明显增多,尤其是胁迫处理3 h时.这一结果为sHSP17.6参与低温锻炼诱导的耐热性提供了更直观的细胞学证据.

关键词: 葡萄幼苗, 低温锻炼, sHSP17.6, 胶体金免疫电镜定位

Abstract: In order to invest igate the protect ing role of small heat shock protein 17. 6( sHSP17. 6) in the heat tolerance induced by cold acclimat ion( CA),and then to explore the mechanism of cross adaptation to different temperature stresses in grape plants. Leaves from two-year-old young grape ( Vitis vinif era L. cv. Jingxiu) plants were used as exper-imental materials.The subcellular localizations of sHSP17. 6 within mesophyll cells were observed using immuno-gold labeling electron microscopic technique. The results showed that CA pretreatment could enhance the expression level of sHSP17. 6 within heat stress. Compared to non-acclimated plants, the density of sHSP17. 6 gold particles was obviously increased in the nucleus and chloroplast of the CA leaves, especially 3 h after heat stress. Which offered the cytological evidence of sHSP17. 6 was contributed to the heat tolerance induced by CA pretreatment in grape plants.

Key words: Young grape plants, Cold acclimation, sHSP17. 6, Immuno-gold electron microscopic localization

中图分类号:

S663.1

张俊环, 黄卫东. 低温锻炼诱导葡萄幼苗的耐热性过程中sHSP17.6在叶片中的亚细胞定位[J]. 华北农学报, 2009, 24(2): 8-11. doi: 10.7668/hbnxb.2009.02.002.

ZHANG Jun-huan, HUANG Wei-dong. Cold Acclimation Induce the Changes in the Subcellular Distribution of Small Heat Shock Protein 17.6 in Young Grape Leaves During Heat Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 8-11. doi: 10.7668/hbnxb.2009.02.002.

http://www.hbnxb.net/CN/Y2009/V24/I2/8

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低温锻炼诱导葡萄幼苗的耐热性过程中sHSP17.6在叶片中的亚细胞定位

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低温锻炼诱导葡萄幼苗的耐热性过程中sHSP17.6在叶片中的亚细胞定位

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