摘要： 以3个来自国外的石刁柏品种(C.IL,PURPLE和APOLLO)为试材,研究不同基因型以及培养基中添加2,4-D、KT、6-BA和NAA等生长物质对石刁柏幼胚胚状体发生的影响。结果表明,幼胚在MS+2,4-D1.0mg/L+KT0.5mg/L+6-BA2.0mg/L培养基中可直接诱导产生体细胞胚。降低2,4-D的浓度,将诱导产生的体细胞胚组织块继代培养2~3次可诱导产生出米黄色颗粒状的胚性愈伤组织,继而有胚状体的发生。胚性细胞继续分裂形成多细胞原胚,经球形胚、梨形胚、长形胚、子叶分化期到成熟胚,其发生的胚状体与单子叶合子胚形成具有相似的过程。
关键词:
石刁柏,
组织培养,
体细胞胚发生,
组织细胞学
Abstract: Three varieties( C. IL, PURPLE and APOLLO) were applied in somatic embryogenesis of Asparagus off icialis L. Different growth substances such as 2, 4-D, KT, 6-BA and NAA were added in theMS substrate.Results indicated that somatic embryos were induced directly from immature embryo axes cultured on basal MS medium supplemented with 2, 4-D 1. 0mg/ L, KT 015 mg/ L and 6-BA 210 mg/L1When decreasing the level of 2, 4-D, yellow embryogenic ca-l lus were induced from tissue of somatic embryos cultured for 2- 3 times, and somatic embryos at different stages appeared1The embryogenic cell first divided into multicellular proembryos, then globular embryoid, pearshaped embryoid, long embryoid, scutiform embryoid and mature embryoid. Asparagus off icialis L. embryogenesis from epidermical cells or subepidermical cells was similar to that of the zygotic embryo.
Key words:
Asparagus off icinalis L.,
Tissue culture,
Somatic embryogenesis,
Histocytology
中图分类号:
DINGXin, FANShuang-xi, GUJian-tian, GAOXia-hong, GAOJie, WANGJun-juan. Studies on Somatic Embryogenesis and Cell Morphology of ImmatureAsparagus off icinalis L. Embryos[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.047.