摘要： 采用不同的萃取剂从甘草的根、茎、叶、草药和甘草悬浮培养细胞中萃取甘草酸。结果表明,选择含氨0.5%、浓度为10%的乙醇溶液作为萃取剂,萃取得到的甘草酸粗品含量均高于50%乙醇萃取的萃取含量,检测出在优选甘草悬浮培养细胞中所含的甘草酸铵的含量高于其他材料,其中通过细胞悬浮培养,获得的甘草酸铵含量最高可达160.81mg/g。
关键词:
甘草悬浮培养细胞,
萃取剂,
甘草酸
Abstract: By different extractant , glycyrrihizic acid can be extracted from glycyrrihizic roots , stems , leaves , herbalmedicine and Glycyrrihizic suspension culture cells .The crude content of glycyrrihizic acid extracted by 10 %ethanol containing0.5 % ammonia was higher than that extracted by 50 % ethanol .The results showed the content of inammoniumglycyrrhetate optimized glycyrrihizic suspension culture cells were higher than that in other materials .And also by cellsuspension culture , the maximum content of glycyrrihizic acid can reach 160.81 mg/g .
Key words:
Glycyrrihizic suspension culture cell,
Extractant,
Glycyrrihizic acid
中图分类号:
LINan, GAOGuang-rui, WEIJing-fang, LIDong-jie, LIUYing, XUEZhi-zhong. Detection of Glycyrrihizic Acid in Glycyrrihizic Suspension Culture Cell and Herbal Medicine ,Plant[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.048.