高温、强光胁迫对苹果果皮组织APX活性的影响

doi:10.7668/hbnxb.2008.06.033

摘要/Abstract

摘要： 通过对自然条件下苹果树冠不同方位和不同日烧程度果实APX活性的比较，以及在果实上施用外源抗氧化剂，研究了高温和强光胁迫下果实APX活性的变化规律。结果表明：树冠不同方位果实APX活性有明显差异，树冠西南、南和西面三个方位果实表皮组织APX活性均极显著地高于其他方位，这与自然条件下不同方位果实对高温和强光的适应性有直接关系。在一定高温胁迫范围内，APX活性随果实日烧程度增强而上升，但超过一定限度，其活性显著下降，说明过度高温和强光下APX本身也受到伤害。果实喷施不同外源抗氧化剂能提高APX活性，其中以SA处理效果最显著，比对照提高了53.87%。由此可见，适当施用外源抗氧化剂可提高果实APX的活性，从而提高果实的抗氧化能力。

关键词: 高温, 强光, 苹果, 果实, 氧化胁迫

Abstract: The changing patterns of APX act ivity in fruit were examined under high temperature and excessive-light stress in a comparison of APX activity among fruits on different exposures of a canopy and also among different degrees of sunburned fruits,as well as by application with exogenous antioxidative formulations.The results indicated that there were very signif icant differences in APX activity among fruits on different exposures of a canopy with higher activity appearing in SW,S and W fruits,which was associated directly with fruit accclimation to high temperature and excessive light intensity under natural conditions.Within a certain range,APX activity increased with the degrees of sunburned fruits but decreased abruptly beyond a threshold limit,implying that APX was damaged itself as a result of extremely high temperature or excessive sunlight.Spraying fruits with different types of exogenous ant ioxidative formulat ions could improve APX activity,among which SA showed the best effect by a rise of more than 5 times,compared to the control.Therefore,it was concluded that spraying fruits with exogenous antioxidants could raise their APX activity,thus improving fruit antioxidative capability.

Key words: High temperature, Excessive light, Apple, Fruit, Oxidative stress

中图分类号:

S661.01

王静璞, 李英丽, 张建光. 高温、强光胁迫对苹果果皮组织APX活性的影响[J]. 华北农学报, 2008, 23(6): 144-147. doi: 10.7668/hbnxb.2008.06.033.

WANG Jing-pu, LI Ying-li, ZHANG Jian-guang. Effect of High-temperature and Excessive-light Stress on APX Activity in Apple Peel[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 144-147. doi: 10.7668/hbnxb.2008.06.033.

http://www.hbnxb.net/CN/Y2008/V23/I6/144

参考文献

[1] Bas van de Ende.Sunburn management[J].Compact Fruit Tree,1999,32(1):13-14.
[2] Ball L,Accotto G P,Bechtold U,et al.Evidence for a direct link between glutathione biosynthesis and stress defense gene expression in Arabidopsis[J].Plant Cell,2004,16:2448-2462.
[3] Andrews P K,Johnson J R.Physiology of sunburn development in apples[J].Good Fruit Grower,1996,47(12):33-36.
[4] 朱为民,丁海东,齐乃敏,等.Cd2+胁迫对番茄幼苗抗坏血酸-谷胱甘肽循环代谢的影响[J].华北农学报,2005,20(3):50-53.
[5] 杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121-126.
[6] 孙卫红,王伟青,孟庆伟.植物抗坏血酸过氧化物酶的作用机制、酶学及分子特性[J].植物生理学通讯,2005,41(2):143 -147.
[7] 苏维埃.植物对温度逆境的适应[M]//余叔文,汤章城.植物生理与分子生物学.北京:科学出版社,2000:731-732.
[8] Panchuk I I,Volkov R A,Schoffl F.Heat stress-and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis[J].Plant Physiology,2002,129(2):838-853.
[9] Ma F,Cheng L.Exposure of the shaded side of apple fruit to full sun leads to up-regulation of both the xanthophyll cycle and the ascorbate-glutathione cycle[J].Plant Science,2004,166:1479-1486.
[10] Ma F,Cheng L.The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate-glutathione pathway than the shaded peel[J].Plant Science,2003,165:819-827.
[11] Chaitanya K V,Sundar D,Masilamani S,et al.Variation in heat stress-induced antioxidant enzyme activities among three mulberry cultivars[J].Plant Growth Regulation,2002,36(2):175-180.
[12] Xu Q,Huang B.Antioxidant metabolism associated with summer leaf senescence and turf quality decline for creeping bentgrass[J].Crop Science,2004,44(2):553-560.
[13] Larkindale J,Huang B.Thermotolerance and antioxidant systems in Agrostis stolonifera:involvement of salicylic acid,abscisic acid,calcium,hydrogen peroxide,and ethylene[J].Journal of Plant Physiology,2004,161(4):405-413.
[14] Dash S,Mohanty S.Response of seedlings to heat-stress in cultivars of wheat:growth temperature-dependent differential modulation of photosystem 1 and 2 activity,and foliar antioxidant defense capacity[J].Journal of Plant Physiology,2002,159(1):49-59.
[15] Gechev T,Willekens H,Montagu M.Different responses of tobacco antioxidant enzymes to light and chilling stress[J].Journal of Plant Physiology,2003,160(5):509-515.
[16] Vacca R A,De Pinto M C,Valenti D,et al.Production of reactive oxygen species,alteration of cytosolic ascorbate peroxidase,and impairment of mitochondrial metabolism are early events in heat shock-induced programmed cell death in tobacco bright-yellow 2 cells[J].Plant Physiology,2004,134(3):1100-1112.
[17] 白团辉,马锋旺,李翠英,等.水杨酸对根际低氧胁迫八棱海棠幼苗活性氧代谢的影响[J].园艺学报,2008,35(2):163-168.
[18] 张健强,张建光,付艾莉,等.外源抗氧化剂对高温胁迫下苹果果皮组织酶活性的影响[J].华北农学报,2005,20(2):41-44.
[19] Comparot S M,Graham C M,Reid D M.Methyl jasmonate elicits a differential antioxidant response in light-and dark_grown canola(Brassica napus)roots and shoots[J].Plant Growth Regulation,2002,38(1):21-30.
[20] Foyer C H.Ascorbate and glutathione:keeping active oxygen under control[J].Annu Rev Plant Physiol,Plant Mol,Biol,1998,49:249-279.
[21] 粟霞,张军科,马锋旺.苹果叶片抗坏血酸过氧化物酶基因cDNA 的克隆[J].西北农林科技大学学报:自然科学版,2007,35(10):77-80.
[22] 李筠,邓西平,郭尚洙,等.转铜/锌超氧化物岐化酶和抗坏血酸过氧化物酶基因甘薯的耐旱性[J].植物生理与分子生物学报,2006,32(4):451-457.
[23] 沈文飚.抗坏血酸过氧化物酶活性测定的探讨[J].植物生理学通讯,1996,32(3):2053-2058.
[24] 张建光,刘玉芳,李英丽,等.光照强度对苹果表面温度变化的影响[J].生态学报,2004(6):197-200.
[25] 张建光,李英丽,邸葆,等.高温、强光胁迫对苹果树冠不同方位果皮的氧化胁迫研究[J].中国农业科学,2004,37(1):1976-1980.
[26] 马春花,马锋旺,李明军,等.外源抗坏血酸对离体苹果叶片衰老过程的影响[J].园艺学报,2006,33(6):1179-1184.
[27] Ishikawa T,Dowdle J,Smirnoff N.Progress inmanipulating ascorbic acid biosynthesis and accumulation in p lants[J].Physiologia Plantarum,2006,126(3):343-355.
[28] Shigeru S,Takahiro I,Masahiro T,et al.Regulation and function of ascorbate peroxidase isoenzymes[J].Journal of Experimental Botany,2002,5:1305-1319.
[29] 马玉华,马锋旺,马小卫,等.干旱胁迫对苹果叶片抗坏血酸含量及其代谢相关酶活性的影响[J].西北农林科技大学学报:自然科学版,2008,36(3):150-160.
[30] Jacek Patykoski,Alina Majczak,Katarzyna Bergier.Ascorbate content and peroxidase activities in apple fruits during storage[J].Journal of Fruit and Ornamental Plant Research,2007,15:21-33.
[31] 马春花,马锋旺,李明军,等.不同叶龄苹果叶片抗坏血酸含量与其代谢相关酶活性的比较[J].园艺学报,2007,34(4):995-998.

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