华北农学报 ›› 2008, Vol. 23 ›› Issue (6): 20-27. doi: 10.7668/hbnxb.2008.06.005

所属专题: 玉米 生物技术

• 论文 • 上一篇    下一篇

玉米产量相关性状QTL通用图谱的构建及功能候选基因的开发

张耿1,2, 祝丽英1, 陈景堂1   

  1. 1. 河北农业大学, 农学院, 河北, 保定, 071001;
    2. 浙江大学, 农业与生物技术学院, 浙江, 杭州, 310029
  • 收稿日期:2008-10-11 出版日期:2008-12-28
  • 通讯作者: 祝丽英(1973-),女,河北南和人,副教授,硕士,主要从事玉米遗传育种研究。
  • 作者简介:张耿(1985-),男,河北邢台人,在读硕士,主要从事植物基因组学研究。
  • 基金资助:
    河北农业大学科技将帅计划;河北农业大学青年基金(qn200613)

Consensus Map of the QTLs Relevant to Yield Traits of Maize and the Development of Functional Candidate Gene

ZHANG Geng1,2, ZHU Li-ying1, CHEN Jing-tang1   

  1. 1. College of Agronomy, Agricultural University of Hebei, Baoding 071001, China;
    2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
  • Received:2008-10-11 Published:2008-12-28

摘要: 通过生物信息学手段,收集整理玉米基因组数据库中产量相关性状的QTL信息,借助高密度玉米遗传连锁图谱IBM 2005 Neighbors和临近分子标记,建立了涵盖干物质和淀粉产量、籽粒相关性状、粒重相关性状以及穗部相关性状四大类共计1个指标的QTL通用图谱。并将位于QTL分布密集区域的基因,与水稻基因序列进行比对,找到了与水稻木质素合成有关的gh2具有高同源性的基因AY10976,同时改进了利用生物信息学手段筛选功能候选基因的路线图。

关键词: 玉米, 生物信息学, 产量性状, QTL, 一致性图谱

Abstract: The information of QTLs relevant to yield trait in the database about maize(Zea mays L.) were collected through bioinformatics tools,and the consensus map was constructed with the aid of high-density genetic linkage map IBM 2005 Neighbors and the adjacent molecular markers to each QTL. The consensus map covered a total of 18 indicators involving four categories traits such as dry matter and starch yield,grain-related traits, seed weight as well as earrelated traits. We also compared the genes distributed in QTL densely covered area with the sequence of genes in rice (Oryza sativa L.)by the means of BLAST,and searched a gene AY109876 which has high homology with gh2,a gene related with the synthesis of lignin of rice. The route of filtrate functional candidate gene by bioinformatics tools has been improved too.

Key words: Maize (Zea mays L.), Bioinformatics, Yield trait, QTL, Consensus map

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引用本文

张耿, 祝丽英, 陈景堂. 玉米产量相关性状QTL通用图谱的构建及功能候选基因的开发[J]. 华北农学报, 2008, 23(6): 20-27. doi: 10.7668/hbnxb.2008.06.005.

ZHANG Geng, ZHU Li-ying, CHEN Jing-tang. Consensus Map of the QTLs Relevant to Yield Traits of Maize and the Development of Functional Candidate Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 20-27. doi: 10.7668/hbnxb.2008.06.005.

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