摘要： 以口蹄疫病毒株OA/58 RNA为模板，反转录并扩增目的cDNA ，然后与pMD18－T载体连接并转化JM109菌株，提取的重组质粒用凝胶电泳、PCR和BamH I、Hind III双酶切法鉴定。分析表明，口蹄疫病毒VP1、VP2、VP3和VP在核苷酸水平上的变异率是无差异的(p>0.05)；而它们在氨基酸水平上的变异率差异显著(p<0.05)。该毒株与20株源于GenBank中的VP3氨基酸序列比较发现其保守区主要位于第1～2、26～35、37～3、5～57、61～122、12～173、175～209、211～220位。运用同源模建，OA/58 VP3蛋白三维空间结构可分为A，B，C 3个结构区域。保守区氨基酸残基在维持VP3蛋白的空间构象和功能方面具有重要作用。

关键词: 口蹄疫病毒, VP3蛋白, 同源模建

Abstract: Foot2and2mouth disease virus strain OA/ 58 RNAs were used as templates for RT2PCR. The amplified cD2 NA products were cloned into pMD182T vectors and transformed into JM109. The recombinant plasmids were ident ified by electrophoresis, PCR, and analysis of tow cleavages with BamH I and Hind III. After analyzing the difference among VP1, VP2, VP3, VP4, at the nucleotide level, the mutation rates of these 4 encoding sequences were no difference (p> 0. 05), however, at the amino acid level, those mutation rates were different (p< 0. 05). The regions of 1- 24th, 26- 35th, 37- 43th, 45- 57th, 61- 122th, 124- 173th, 175- 209th and 211- 220th in VP3 protein most probably are conservat ive. Depending on homology modeling the FMDV strain OA/ 58 VP3 protein, the 3D mold was gained. And this 3D mold was divided into A, B and C regions. This research should be used as an instruction in order to direct the work on FMDV VP3 protein.

Key words: Foot-and-mouth disease virus, VP3 protein, Homology modeling

中图分类号: 

• Q945