摘要： 以 AF/72 RNA 为模板,反转录并扩增目的cDNA,然后与pGEM-T easy 体连接并转化JM109菌株,提取的重组质粒用凝胶电泳、PCR 和EcoR I 酶切法鉴定.应用BIMAS生物学软件对AF/72株结构蛋白VP1的H-2Dd、H-2Kd、 H-2Ld、 A20限制性T细胞表位进行预测,并对不同限制性的T细胞表位进行对比.结果表明,口蹄疫AF/72株结构蛋白VP1长639 bp,编码213个氨基酸.不同限制性的T细胞表位虽然不尽相同,但与已鉴定的T细胞表位相比较,它们之间也有一些类似的、并且可能是通用型的T细胞表位.本试验通过生物信息学方法预测AF/72结构蛋白VP1的T细胞表位,为进一步试验确定T细胞表位和口蹄疫病毒T细胞表位的研究提供了一种有效的方法.同时为口蹄疫病毒表位疫苗的设计提供有效地理论基础.

关键词: 口蹄疫病毒, T细胞表位, VP1蛋白

Abstract: Virus strain AF/72 RNAs were used as templates for RT-PCR. The amplified cDNA products were cloned into pGEM-T easy vectors and transformed into JM109. The recombinant plasmids were identified by electrophoresis,PCR,and EcoR I cleavage. Application of biological software to H-2Dv,H-2Kv,H-2Lv,A20 restricted T cell epitope and the difference of restrictive T-cell epitope were compared. The results showed that the VP1 region of FMDV AF /72 strain was 639 bp in length and codes for a polypeptide of 213 amino acids. Although different restrictive T cell epitope was not identical,but the predicted T cell epitope was similar to compared with identified T cells and might is the universal T-cell. This study prediction of T cell epitope of structural protein of FMDV AF /72 strain through bioinformatics. It would be helpful for identification of T cell epitopes for structural protein of FMDV AF /72 strain using experimental methods and provides an effective method for the virus T cell epitope research simultaneously,for the virus epitope based accines design provides effective theoretical basis.

Key words: FMDV, T cell epitope, VP1 protein

中图分类号: 

• Q78