摘要： 将从Genbank中提出的20株O型FMDV的全基因组分割成12个编码区域,即Lab、VP4、VP2、VP3、VP1、2A、2B、2C、3A、3B、3C和3D。通过方差分析,12个编码序列的相对变异率之间无差异(P>0.05) 而这12种编码序列所对应的氨基酸相对突变率之间差异显著(P>0.01)。利用Duncan法对这12种氨基酸序列相对突变率进行多重比较,发现3A蛋白的氨基酸相对突变率比其余11种蛋白的差异都显著(P> 0.05) VP2、LPro的氨基酸相对突变率与VP4、2A、2C、3Cpro、3DPol之间存在差异(P>0.05) 而VP4、2A、2C、3CPro、3DPol之间无差异。结果显示,O型FMDV自身RNA的转录和机体对FMDV施加的免疫压力不是造成FMDV变异的主要原因,而各种病毒产物的功能选择压力才是FMDV分子进化的主要原因。
关键词:
口蹄疫病毒,
相对变异率,
方差分析,
Duncan法
Abstract: A total of 20 foot-and mouth disease virus (FMDV) serotype O was obtained from C}nbank and was divided into 12 encoding sequences (Lab,V P4,VP2,V P3,V PI,2A,2B,2 C, 3A,3B,3C and 3D).After analng the didference among them by analysis of variance,at the nucleotide level,the relative mutation rates of these 12 encoding se-quences were no difference(P>0.05),however,at the amino acid level,those relative mutation rates were different(P<0. 01).And the analysis of Duncan method indicated that the relative mutation rate of 3A protein is the highest. Compari-son with VP4,3CPro,3DPol,2A,2C,the relative mutation rates of VP2 and LPro were different than them( P<0. 05).Inaddition,the relative mutation rates of VP4,3CPro,3DPol,2A,2C were。different. The proposed results strongly suggestedthat serotype O FMDV RNA transcription and the pressure of immunity failed to play an important role in driving FMDV genome evolution. An evolutionary pressure is related to functional conservation.
Key words:
Foot-and mouth disease virus,
Relative mutation rate,
Analysis of variance,
Duncan method
中图分类号:
周建华, 丛国正, 常惠芸. O型口蹄疫病毒各编码基因及其产物变异率的分析[J]. 华北农学报, 2009, 24(1): 60-63. doi: 10.7668/hbnxb.2009.01.014.
ZHOU Jian-hua, CONG Guo-zheng, CHANG Hui-yun. The Analysis of Products Mutation Rate of Encoding Sequences and Their Products in Serotype O Foot-and-Mouth Disease Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 60-63. doi: 10.7668/hbnxb.2009.01.014.