华北农学报

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栽培亚麻×野生亚麻种间杂交种的真实性鉴定

张丽丽, 白苇, 米君, 曲志华, 乔海明, 李世芳, 李爱荣, 马建富, 郭娜, 刘晶晶   

  1. 河北省张家口市农科院, 河北 张家口 075000
  • 收稿日期:2012-08-10 出版日期:2012-12-31
  • 作者简介:张丽丽(1983 - ), 女, 河北保定人, 助理研究员, 硕士研究生, 主要从事胡麻育种与栽培技术研究。
  • 基金资助:
    现代农业产业技术体系建设专项资金资助项目(nycytx-22);胡麻种间杂交种的鉴定(Y10-4)

Identification Interspecific Hybridization between Cultivated Flax and Wild Flax

ZHANG Li-li, BAI Wei, MI Jun, QU Zhi-hua, QIAO Hai-ming, LI Shi-fang, LI Ai-rong, MA Jian-fu, GUO Na, LIU Jing-jing   

  1. Zhangjiakou Academy of Agriculture Science,Zhangjiakou 075000,China
  • Received:2012-08-10 Published:2012-12-31

摘要: 为进一步证明亚麻种间杂交种的真实性,对前人开发的14对SSR引物进行了再次筛选,对栽培种亚麻与多年生宿根型野生亚麻的种间杂交种和亲本之间的亲子关系进行鉴定。结果显示,7对引物能很好地标记出双亲,并成功标记出了1份杂交种与亲本之间存在亲子关系,鉴定了种间杂交种的真实性。

关键词: 亚麻, 种间杂交, SSR标记, 引物筛选

Abstract: In order to further prove the authenticity of the hybrids, this articule was about the test screenedprimer from the previous 14 SSR primers and identified the parental relationship between the hybrids and parents.The results showed that 7 primers marked the parents, and marked out the parental relationship between hybrid andparents successfully, identified the authenticity of interspecific hybrids.

Key words: Flax, Interspecific hybridization, SSR marker, Screen primer

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引用本文

ZHANG Li-li, BAI Wei, MI Jun, QU Zhi-hua, QIAO Hai-ming, LI Shi-fang, LI Ai-rong, MA Jian-fu, GUO Na, LIU Jing-jing. Identification Interspecific Hybridization between Cultivated Flax and Wild Flax[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.011.

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