华北农学报 ›› 2015, Vol. 30 ›› Issue (S1): 46-50. doi: 10.7668/hbnxb.2015.S1.008

所属专题: 棉花

• 论文 • 上一篇    下一篇

83份早熟抗虫棉种质资源的SSR标记聚类分析

董章辉, 赵丽芬, 赵彦坤, 眭书祥, 张艳丽, 王虎, 李增书, 李爱国, 朱青竹   

  1. 石家庄市农林科学研究院, 河北 石家庄 050041
  • 收稿日期:2015-09-12 出版日期:2015-12-28
  • 通讯作者: 朱青竹(1963-),女,河北石家庄人,研究员,硕士,主要从事棉花育种相关研究.
  • 作者简介:董章辉(1981-),男,河北石家庄人,助理研究员,博士,主要从事棉花遗传育种相关研究.
  • 基金资助:
    国家转基因重大专项(2014ZX08005-002);河北省现代产业技术体系棉田复种新品种选育项目;石家庄市重大专项(131490097A)

Cluster Analysis in 83 Early-maturing Cotton Germplasms by SSR Markers

DONG Zhang-hui, ZHAO Li-fen, ZHAO Yan-kun, SUI Shu-xiang, ZHANG Yan-li, WANG Hu, LI Zeng-shu, LI Ai-guo, ZHU Qing-zhu   

  1. Shijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, China
  • Received:2015-09-12 Published:2015-12-28

摘要: 早熟棉遗传多样性研究是早熟棉遗传改良的基础,对培育早熟棉新品种有重要意义.为了进一步改善现有早熟棉品种的早熟性,为早熟棉育种的亲本组配提供依据,利用35对SSR分子标记,对来自不同地区的83份早熟抗虫棉种质进行遗传多样性分析.结果表明,筛选出的22对多态性引物共扩增出30条多态性条带.NTSYS-pc2.11的遗传多样性分析表明,83份材料的相似系数变化为0.73~0.98,当遗传相似系数为0.73时,将83份棉花早熟材料分为两大类.第Ⅰ类群包含59个材料,第Ⅱ类群包含24个材料.表明我国早熟棉遗传基础比较狭窄,今后育种中要选用亲缘关系较远的材料作为杂交亲本,创制新的早熟棉种质资源.

关键词: 早熟棉, 种质资源, SSR标记, 遗传多样性

Abstract: It is very important to study the genetic diversity of early-maturing cotton for breeding early-maturing cultivar.To improve the prematurity of early-maturing cotton and select right parent combinations,we analysed the genetic diversity of 83 early-maturing cotton germplasms from different ecotypes in China using 35 pairs of SSR primer.As a result,we got 22 pairs of polymorphic primer and 30 allelic variations.The result of NTSYS-pc2.11 analysis indicated that genetic similarity coefficient of the 83 germplasms ranged from 0.73 to 0.98,which showed that there was limited genetic diversity in early-maturing cotton germplasms.The 83 early-maturing cotton germplasms were divided into 2 groups with the genetic similarity coefficient at 0.73,which included 59 and 24 members respectively.We should select parent combinations with distant phylogenetic relationships for early-maturing cotton breeding in future work.

Key words: Early-maturing cotton, Germplasms, SSR marker, Genetic diversity

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引用本文

董章辉, 赵丽芬, 赵彦坤, 眭书祥, 张艳丽, 王虎, 李增书, 李爱国, 朱青竹. 83份早熟抗虫棉种质资源的SSR标记聚类分析[J]. 华北农学报, 2015, 30(S1): 46-50. doi: 10.7668/hbnxb.2015.S1.008.

DONG Zhang-hui, ZHAO Li-fen, ZHAO Yan-kun, SUI Shu-xiang, ZHANG Yan-li, WANG Hu, LI Zeng-shu, LI Ai-guo, ZHU Qing-zhu. Cluster Analysis in 83 Early-maturing Cotton Germplasms by SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 46-50. doi: 10.7668/hbnxb.2015.S1.008.

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