摘要： 转化效率低一直是黄瓜转基因的瓶颈，本研究旨在采用硫辛酸(LA)和乙酰丁香酮(AS)辅助进行农杆菌转化，研究硫辛酸作为一种新的转化诱导剂对黄瓜遗传转化的影响。结果表明，在添加浓度相同的情况下，仅在培养基中添加LA的转化效率比较仅在菌液中加入LA有显著提高，而当菌液和培养基中同时加入LA与AS的转化频率最高，即采用100 mg/L LA和50 mg/L AS处理后，黄瓜子叶节Hyg抗性芽的再生频率由28.3%升高到86.7%，SouthernBlot验证抗性植株的阳性率为7.5%。因此，在黄瓜遗传转化过程中使用硫辛酸有利于转化细胞的分化，更有助于提高抗性植株的阳性率。

关键词: 硫辛酸, 根癌农杆菌, 遗传转化, 黄瓜, 子叶节

Abstract: It's difficult to increase the transformation efficiency in cucumber． The effect of LA and AS duringtransformation of cucumber cotyledon node was investigated． The plant regenerated efficiency of cucumber was significantly increased from 28． 3 % to 86． 7% ， the transformation efficiency was 7． 5% analyzed by Southern-blotwhen 100 mg /L LA and 50 mg /L AS added in culture media and Agrobacterium liquid． Including lipoic acid in culture media during Agrobacterium transformation processes of cucumber can improve the transformation method． LAapplication in transformation has enabled the resolution of problems in cucumber: difficulty in regenerating transformed tissues and shoot escapes that limit the number of transgenic plants．

Key words: Lipoic acid, Agrobacterium tumefaciens, Transformation, Cucumber, Cotyledon node．

中图分类号: 

• S642.2