摘要： 为了解MADS-box蛋白中AGL6亚家族基因ZAG3在玉米花发育中的功能,对ZAG3基因进行序列分析显示,该基因开放阅读框为768 bp,编码255个氨基酸的MADS-box蛋白。亚细胞定位预测显示,ZAG3定位于细胞核中。启动子预测分析表明,ZAG3基因启动子区具有启动子的核心组件、分生组织表达的组件和脱落酸、赤霉素等植物激素应答组件,但没有与茉莉酸相关的应答组件。此外,实时荧光定量PCR表达分析显示,喷施茉莉酸甲酯可以使ZAG3基因在玉米雄穗中的表达明显提高,暗示了该基因与JA介导的雄穗性别决定过程是有关系的。

关键词: 玉米, 性别决定, AGL6, ZAG3, 茉莉酸

Abstract: In this paper,bioinformatical and expression analysis were used to examine the functions of ZAG3,a AGL6-like gene in maize.Sequence analysis indicated that ZAG3 encoded a MADS-box protein with 255 amino acids.ZAG3 protein was predicted to be subcellular located in the nucleus.Furthermore,the promoter sequenced was analysised to contain some important regulatory element,including core promoter elements,meristem expression element,abscisic acid responsiveness and gibberellin-responsiveness element,etc.Noticeable,MeJA-responsive regulatory element was not found in the promoter region,whereas the expression of ZAG3 was remarkable up-regulated in the MeJA-treated tassel by using the Q-PCR technology.These results suggest that ZAG3 gene may relate to the JA-mediated sex determination process in maize tassel.

Key words: Maize, Sex determination AGL6 ZAG3 JA

中图分类号: 

• S513