华北农学报 ›› 2012, Vol. 27 ›› Issue (1): 118-123. doi: 10.3969/j.issn.1000-7091.2012.01.022

所属专题: 水稻

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低温胁迫下不同磷营养对水稻叶片质膜透性及抗氧化酶活性的影响

侯立刚1,2, 陈温福1, 马巍2, 赵国臣2, 齐春艳2, 刘亮2, 孙洪娇2   

  1. 1. 沈阳农业大学水稻研究所, 辽宁沈阳 100866;
    2. 吉林省农业科学院水稻研究所, 吉林公主岭 136100
  • 收稿日期:2011-11-08 出版日期:2012-02-28
  • 通讯作者: 陈温福(1955- ), 男, 辽宁法库人, 教授, 博士, 主要从事水稻高产育种与栽培技术研究。
  • 作者简介:候立刚(1974- ), 男, 吉林梨树人, 研究员, 博士, 主要从事水稻耕作栽培研究。
  • 基金资助:
    农业部农业科研专项项目(200903003);吉林省现代农业技术体系项目(2009-2010);科技部丰粮工程项目(2011BAD16B10)

Effects of Different Phosphate Fertilizer Application on Permeability of Membrane and Antioxidative Enzymes in Rice under Low Temperature Stress

HOU Li-gang1,2, CHEN Wen-fu1, MA Wei2, ZHAO Guo-chen2, QI Chun-yan2, LIU Liang2, SUN Hong-jiao2   

  1. 1. Rice Research Institute, Shenyang Agricultural University, Shenyang 100866, China;
    2. Rice Research Institute, Jilin Academy of Agriculture Sciences, Gongzhuling 136100, China
  • Received:2011-11-08 Published:2012-02-28

摘要: 采用盆栽试验的方法,以耐冷性品种吉粳81和非耐冷性品种长白9为供试材料,通过不同的磷肥施用量,研究低温胁迫下磷营养对水稻质膜透性及抗氧化酶活性的影响。结果表明:施磷后,随着施磷量的增加,2个水稻品种叶片电解质外渗率和丙二醛含量均先降低后升高,而可溶性糖、脯氨酸含量、过氧化物酶(POD) 、过氧化氢酶(CAT) 、超氧化物歧化酶(SOD) 的活性则呈先升后降的趋势。通过对比常温对照发现,低温下适当增磷可明显提高2水稻品种叶片可溶性糖、脯氨酸含量及3种抗氧化酶活性的上升幅度,减缓电解质外渗率和MDA含量的下降幅度。研究还表明,低温增磷对提升非耐冷品种长白9叶片可溶性糖、脯氨酸含量和3种抗氧化酶活性,降低电解质外渗率和丙二醛含量效果更明显。因此,通过体外增施磷肥可明显提高叶片质膜透性及抗氧化酶活性,从而有利于提高水稻的抗寒性能力。

关键词: 低温胁迫, 磷肥, 水稻, 质膜透性, 抗氧化酶活性

Abstract: A pot experiment was conducted to investigate the effects of different level of phosphate fertilizer application on permeability of membrane and antioxidative enzymes in rice(non-chilling cv. Changbai 9 and chillingtolerant cv. Jijing 81) under low temperature stress. The results showed that as the increasing of P fertilizer application, membrane permeability and MDA decreased and the increased in P-treated rice, but soluble sugar content, proline, peroxidase(POD), catalase(CAT) and superoxide dismutase(SOD) increased and then decreased. Compared with the normal temperature treatment, appropriately increasing phosphorus can improve the content of soluble sugar, praline, add the activity of POD, CAT, SOD, and decline membrane permeability and MDA content in rice under low temperture stress. The increase rates of the soluble sugar, proline, POD, CAT, SOD for P treatment in non-chilling cv. Changbai 9 were higher than those in chilling-tolerant cv. Jijing 81 under low temperature stress. and membrane permeability and MDA content decreased even more. The results indicated that membrane permeability and antioxidative enzymes of rice can be improved by exogenous application of phosphorus, it is beneficial to increase the cold resistance of rice.

Key words: Low temperature stress, Phosphate fertilizer, Rice, Permeability of membrane, Antioxidative enzymes

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引用本文

侯立刚, 陈温福, 马巍, 赵国臣, 齐春艳, 刘亮, 孙洪娇. 低温胁迫下不同磷营养对水稻叶片质膜透性及抗氧化酶活性的影响[J]. 华北农学报, 2012, 27(1): 118-123. doi: 10.3969/j.issn.1000-7091.2012.01.022.

HOU Li-gang, CHEN Wen-fu, MA Wei, ZHAO Guo-chen, QI Chun-yan, LIU Liang, SUN Hong-jiao. Effects of Different Phosphate Fertilizer Application on Permeability of Membrane and Antioxidative Enzymes in Rice under Low Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 118-123. doi: 10.3969/j.issn.1000-7091.2012.01.022.

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