摘要： 为了研究转BADH脱氢酶基因水稻的抗旱性，以受体亲本中花8和水稻旱作品种开系7及陆稻品种白珍珠作对照，于塑料营养钵中培养幼苗至五叶期进行干旱胁迫，测定了转BADH基因水稻品系52-7，51-15幼苗的生理生化变化。结果表明，干旱胁迫下，水稻幼苗细胞膜透性增加，膜脂过氧化产物MDA含量以及氨基酸、可溶性糖和游离脯氨酸含量提高，可溶性蛋白质含量降低，POD活性提高。但52-7和51-15细胞膜透性较小，MDA积累较少，蛋白质、氨基酸含量的变化幅度较小，可溶性糖、游离脯氨酸含量的增加幅度较大，POD和SOD活性较高。52-7，51-15与中花8具有相同的POD同工酶谱，与开系7和白珍珠有差异。干旱胁迫诱导中花8产生3条新酶带而与开系7和白珍珠相同，其他各品种酶谱不变。52-7和51-15的抗旱性强于对照品种。

关键词: 水稻, BADH基因, 抗旱性

Abstract: Rice seedlings of the varieties transferred BADH gene 52-7 and 51-15 were potted in plastic basins to study their anti-drought ability, when they grew to 5 leaves, they were intimidated in drought, and then some physiological and biological changes of them were tested. The rice seedlings of BADH gene receiver Zhonghua 8 and dry planting variety Kaixi 7 and the variety of land rice Baizhenzhu were regarded as contrasts. The result showed that the membrane permeability of rice seedlings increased under drought stress, the contents of MDA and amino acid, soluble sugar and free proline increased as well as the activity of POD, while the content of soluble proteins decreased. But the membrane permeability of 52-7 and 51-15 was smaller than that of the other's, and the accumulation of MDA decreased, the change extents of protein and amino acid contents were smaller, the increasing extents of soluble sugar and free proline were bigger, the activities of POD and SOD were higher than those of the other's. There were the same POD isozyme chart among 52-7, 51-15 and Zhonghua 8, but they had some differences with Kaixi 7 and Baizhenzhu. Zhonghua 8 was induced to synthesize 3 kinds of POD isozymes by the treatment of drought stress, it had the same POD isozyme chart with Kaixi 7 and Baizhenzhu' accordingly, the other varieties had no changes for POD isozymes under drought stress. 52-7 and 51-15 had better traits of drought resistance than the other varieties.

Key words: Rice, BADH gene, Anti-drought

中图分类号: 

• S511.01