华北农学报 ›› 2006, Vol. 21 ›› Issue (6): 33-36. doi: 10.3321/j.issn:1000-7091.2006.06.009

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六倍体小黑麦种质资源遗传多样性分析

周福平, 孙黛珍, 王曙光, 李瑞, 冀彩萍   

  1. 山西农业大学农学院, 山西太谷 030801
  • 收稿日期:2006-04-13 出版日期:2006-12-28
  • 作者简介:周福平(1979- ),男,山西交口人,在读硕士,主要从事小麦遗传育种研究。
  • 基金资助:
    山西省青年基金项目(20031044);山西省留学基金项目(2004044);山西农业大学创新基金项目(200142)

Characterizing the Genetic Diversity of a Group of Hexaploid Triticale

ZHOU Fu-ping, SUN Dai-zhen, WANG Shu-guang, LI Rui, JI Cai-ping   

  1. College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
  • Received:2006-04-13 Published:2006-12-28

摘要: 利用RAPD标记对10份六倍体小黑麦、1份普通小麦和1份黑麦种质资源遗传多样性进行了分析,19个RAPD引物中,有10个引物(52.63%)可扩增出清晰且具多态性的条带。10个引物共产生385条DNA片段,其中384条具有多态性,多态性比率为99.74%,不同引物扩增带数变幅为8~71条,平均为38.5条,扩增产物的片段大小为298~3054 bp,材料间的遗传距离变化范围在0.724~0.833之间。对12份供试材料的遗传关系进行聚类分析,在平均GD值0.77水平上可将12份供试材料分为3类。研究结果为将六倍体小黑麦的优良基因导入到普通小麦中提供了理论依据。

关键词: 六倍体小黑麦, RAPD标记, 遗传多样性, 聚类分析

Abstract: The genetic diversity and genetic relationships among ten accessions of Hexaploid Triticale and one accession of wheat and rye were evaluated by RAPD markers.Totally 385 bands were detected with 10 primers,with the average of 38.5.384 polymorphic bands were detected,representing of 99.74% of polymorphic bands(PPB).8 to 71 polymorphic bands were generated by each primer.Among 12 material,the RAPD-based genetic distance(RAMP-GD) ranged from 0.724 to 0.833,with a mean of 0.77.UPGMA cluster analysis showed that these accessions could be divided into three groups.The results provide a theoretical basis for transferring fine genes from Hexaploid Triticale to wheat.

Key words: Hexaploid Triticale, RAPD marker, Genetic diversity, Cluster analysis

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引用本文

周福平, 孙黛珍, 王曙光, 李瑞, 冀彩萍. 六倍体小黑麦种质资源遗传多样性分析[J]. 华北农学报, 2006, 21(6): 33-36. doi: 10.3321/j.issn:1000-7091.2006.06.009.

ZHOU Fu-ping, SUN Dai-zhen, WANG Shu-guang, LI Rui, JI Cai-ping. Characterizing the Genetic Diversity of a Group of Hexaploid Triticale[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(6): 33-36. doi: 10.3321/j.issn:1000-7091.2006.06.009.

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