摘要： 通过研究不同生长素和细胞分裂素组合配比对火鹤、红宝石喜林芋分化和生根的影响,确定了最佳继代、壮苗和生根培养基,并针对火鹤选择出了优良的快繁无性系作为快繁材料。探讨了恒温与变温培养对愈伤组织鲜重增殖分化壮苗的影响。试管苗移栽驯化试验结果表明,只有在基质温度达15～20℃,室温22～25℃时试管苗才能正常生长。还确定了适宜的移栽基质。试验表明以上2种植物皆可在瓶外生根。
关键词:
火鹤,
红宝石喜林芋,
组织培养,
移栽驯化
Abstract: Mordern ornamental plants, Anthurium scherzerianum Schott and Philodendron enbesoens cv.Red Emerald were propagated successfully by means of tissue culture techniques.Through gradual subculture,the best clone was selected as proliferatative material from a large amount of callus of Anthurium scherzerianum schott.The results of transplantation showed that:plantlets could start to grow vigorously only when the temperature of medium in the container was about 15～20 ℃,and room temperature was 22～25 ℃ in all acclimatization stages.Better the transplantation medium of each kind of plant was chosen in this study.Out test tube rooting of cuttings derived from in Rapid Propagation have been achieved by the treatment of exogenous auxin.
Key words:
Anthurium scherzerianum schott,
Philodendron enbesoeus cv.Red Emerald,
Tissue Culture Transplantation
中图分类号:
王利民, 王献革, 李军英, 李静, 张红梅. 观赏植物火鹤、红宝石喜林芋的快速繁殖[J]. 华北农学报, 2002, 17(S1): 213-218. doi: 10.3321/j.issn:1000-7091.2002.z1.047.
WANG Lin-min, WANG Xian-ge, LI Jun-ying, LI Jing, ZHANG Hong-mei. Tissue Cluture and Rapid Propagation of Anthurium scherzrianum and Philodendron enbesoens cv Red Emerald of Modern Ornamental Aroid[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(S1): 213-218. doi: 10.3321/j.issn:1000-7091.2002.z1.047.