摘要： 试验研究了大豆种子恒温（30℃）抽真空密封贮存不同时间后与其活力相应的生理生化和农艺性状指标的变化。结果表明：随种子贮存时间的延长，发芽率（GP）、芽鲜重（BFW）、出苗率（SEP）、株高、单株粒重、外渗量和脱氢酶活性降低，出苗天数和开花天数推迟，过氧化物酶谱带减少，其测定指标基本上都存在“三降点”（起始下降点、显著下降点和临界下降点）。综合分析显示：80%发芽率可考虑作为大豆种子繁殖更新生活力标准，73%~8%之间所在的发芽率范围可考虑作为大豆种子繁殖更新阈值，73%可考虑作为基因库大豆种子繁殖更新的发芽率下限。

关键词: 基因库, 大豆, 活力, 繁殖更新, 阈值

Abstract: The physiologic and biochemical parameters of the soybean seeds and their agronomic characters were investigated after pretreatment with hermetically sealed and vacuumed storage for differential time under 30 ℃.The results are as follows:(1)The GP,BFW,and SEP of the soybean seeds were decreased;the seedling days,and flowering days were delayed;the strain height and weight of seed per plant were decreased;the seed leakage was increased;the activity of dehydrogenase was declined and the numbers of peroxidase isozyme patterns was decreased with prolongation of storage time.(2)The changes of all above parameters had three critical levels in the rapid declining phase.These findings suggest that from 73% to 86% of the GP in the soybean seeds should be taken as an important reference of regeneration threshold;and the seeds which viability declined to 73% must be regenerated.

Key words: Soybean, Regeneration, Viability threshold, Genebank

中图分类号: 

• S55.102